Novel Exons and Splice Variants in the Human Antibody Heavy Chain Identified by Single Cell and Single Molecule Sequencing

Antibody heavy chains contain a variable and a constant region. The constant region of the antibody heavy chain is encoded by multiple groups of exons which define the isotype and therefore many functional characteristics of the antibody. We performed both single B cell RNAseq and long read single m...

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Detalles Bibliográficos
Autores principales: Vollmers, Christopher, Penland, Lolita, Kanbar, Jad N., Quake, Stephen R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4303433/
https://www.ncbi.nlm.nih.gov/pubmed/25611855
http://dx.doi.org/10.1371/journal.pone.0117050
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author Vollmers, Christopher
Penland, Lolita
Kanbar, Jad N.
Quake, Stephen R.
author_facet Vollmers, Christopher
Penland, Lolita
Kanbar, Jad N.
Quake, Stephen R.
author_sort Vollmers, Christopher
collection PubMed
description Antibody heavy chains contain a variable and a constant region. The constant region of the antibody heavy chain is encoded by multiple groups of exons which define the isotype and therefore many functional characteristics of the antibody. We performed both single B cell RNAseq and long read single molecule sequencing of antibody heavy chain transcripts and were able to identify novel exons for IGHA1 and IGHA2 as well as novel isoforms for IGHM antibody heavy chain.
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spelling pubmed-43034332015-01-30 Novel Exons and Splice Variants in the Human Antibody Heavy Chain Identified by Single Cell and Single Molecule Sequencing Vollmers, Christopher Penland, Lolita Kanbar, Jad N. Quake, Stephen R. PLoS One Research Article Antibody heavy chains contain a variable and a constant region. The constant region of the antibody heavy chain is encoded by multiple groups of exons which define the isotype and therefore many functional characteristics of the antibody. We performed both single B cell RNAseq and long read single molecule sequencing of antibody heavy chain transcripts and were able to identify novel exons for IGHA1 and IGHA2 as well as novel isoforms for IGHM antibody heavy chain. Public Library of Science 2015-01-22 /pmc/articles/PMC4303433/ /pubmed/25611855 http://dx.doi.org/10.1371/journal.pone.0117050 Text en © 2015 Vollmers et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Vollmers, Christopher
Penland, Lolita
Kanbar, Jad N.
Quake, Stephen R.
Novel Exons and Splice Variants in the Human Antibody Heavy Chain Identified by Single Cell and Single Molecule Sequencing
title Novel Exons and Splice Variants in the Human Antibody Heavy Chain Identified by Single Cell and Single Molecule Sequencing
title_full Novel Exons and Splice Variants in the Human Antibody Heavy Chain Identified by Single Cell and Single Molecule Sequencing
title_fullStr Novel Exons and Splice Variants in the Human Antibody Heavy Chain Identified by Single Cell and Single Molecule Sequencing
title_full_unstemmed Novel Exons and Splice Variants in the Human Antibody Heavy Chain Identified by Single Cell and Single Molecule Sequencing
title_short Novel Exons and Splice Variants in the Human Antibody Heavy Chain Identified by Single Cell and Single Molecule Sequencing
title_sort novel exons and splice variants in the human antibody heavy chain identified by single cell and single molecule sequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4303433/
https://www.ncbi.nlm.nih.gov/pubmed/25611855
http://dx.doi.org/10.1371/journal.pone.0117050
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