Chromatin Interaction Analysis with Paired-End Tag (ChIA-PET) sequencing technology and application

BACKGROUND: Long-range chromatin interactions play an important role in transcription regulation. Chromatin Interaction Analysis with Paired-End-Tag sequencing (ChIA-PET) is an emerging technology that has unique advantages in chromatin interaction analysis, and thus provides insight into the study...

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Detalles Bibliográficos
Autores principales: Li, Guoliang, Cai, Liuyang, Chang, Huidan, Hong, Ping, Zhou, Qiangwei, Kulakova, Ekaterina V, Kolchanov, Nikolay A, Ruan, Yijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4303937/
https://www.ncbi.nlm.nih.gov/pubmed/25563301
http://dx.doi.org/10.1186/1471-2164-15-S12-S11
Descripción
Sumario:BACKGROUND: Long-range chromatin interactions play an important role in transcription regulation. Chromatin Interaction Analysis with Paired-End-Tag sequencing (ChIA-PET) is an emerging technology that has unique advantages in chromatin interaction analysis, and thus provides insight into the study of transcription regulation. RESULTS: This article introduces the experimental protocol and data analysis process of ChIA-PET, as well as discusses some applications using this technology. It also unveils the direction of future studies based on this technology. CONCLUSIONS: Overall we show that ChIA-PET is the cornerstone to explore the three-dimensional (3D) chromatin structure, and certainly will lead the forthcoming wave of 3D genomics studies.

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