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Hypertonic saline releases the attached small intestinal cystic fibrosis mucus

Hypertonic saline inhalation has become a cornerstone in the treatment of cystic fibrosis (CF), but its effect on CF mucus is still not understood. In CF, mucus stagnates in the airways, causing mucus plugging, and forming a substrate for bacterial invasion. Using horizontal Ussing-type chambers to...

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Detalles Bibliográficos
Autores principales: Ermund, Anna, Meiss, Lauren N, Scholte, Bob J, Hansson, Gunnar C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4303949/
https://www.ncbi.nlm.nih.gov/pubmed/25311799
http://dx.doi.org/10.1111/1440-1681.12322
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author Ermund, Anna
Meiss, Lauren N
Scholte, Bob J
Hansson, Gunnar C
author_facet Ermund, Anna
Meiss, Lauren N
Scholte, Bob J
Hansson, Gunnar C
author_sort Ermund, Anna
collection PubMed
description Hypertonic saline inhalation has become a cornerstone in the treatment of cystic fibrosis (CF), but its effect on CF mucus is still not understood. In CF, mucus stagnates in the airways, causing mucus plugging, and forming a substrate for bacterial invasion. Using horizontal Ussing-type chambers to allow easy access to the tissue, we have recently shown that the small intestinal mucus of CF mice is attached to the epithelium and not freely movable as opposed to normal mucus, thus pointing to a similarity between the CF mucus in the ileum and airways. In the same type of system, we investigated how hypertonic saline affects mucus thickness, attachment and penetrability to fluorescent beads the size of bacteria in ileal explants from the cystic fibrosis transmembrane conductance regulator mutant (ΔF508) mouse, in order to characterize how this common therapy affects mucus properties. Hypertonic saline (1.75–5%) detached the mucus from the epithelium, but the mucus remained impenetrable to beads the size of bacteria. This approach might be used to test other mucolytic interventions in CF.
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spelling pubmed-43039492015-02-02 Hypertonic saline releases the attached small intestinal cystic fibrosis mucus Ermund, Anna Meiss, Lauren N Scholte, Bob J Hansson, Gunnar C Clin Exp Pharmacol Physiol Original Articles Hypertonic saline inhalation has become a cornerstone in the treatment of cystic fibrosis (CF), but its effect on CF mucus is still not understood. In CF, mucus stagnates in the airways, causing mucus plugging, and forming a substrate for bacterial invasion. Using horizontal Ussing-type chambers to allow easy access to the tissue, we have recently shown that the small intestinal mucus of CF mice is attached to the epithelium and not freely movable as opposed to normal mucus, thus pointing to a similarity between the CF mucus in the ileum and airways. In the same type of system, we investigated how hypertonic saline affects mucus thickness, attachment and penetrability to fluorescent beads the size of bacteria in ileal explants from the cystic fibrosis transmembrane conductance regulator mutant (ΔF508) mouse, in order to characterize how this common therapy affects mucus properties. Hypertonic saline (1.75–5%) detached the mucus from the epithelium, but the mucus remained impenetrable to beads the size of bacteria. This approach might be used to test other mucolytic interventions in CF. Blackwell Publishing Ltd 2015-01 2014-12-22 /pmc/articles/PMC4303949/ /pubmed/25311799 http://dx.doi.org/10.1111/1440-1681.12322 Text en © 2014 The Authors. Clinical and Experimental Pharmacology and Physiology published by Wiley Publishing Asia Pty Ltd. http://creativecommons.org/licenses/by-nc/4.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Ermund, Anna
Meiss, Lauren N
Scholte, Bob J
Hansson, Gunnar C
Hypertonic saline releases the attached small intestinal cystic fibrosis mucus
title Hypertonic saline releases the attached small intestinal cystic fibrosis mucus
title_full Hypertonic saline releases the attached small intestinal cystic fibrosis mucus
title_fullStr Hypertonic saline releases the attached small intestinal cystic fibrosis mucus
title_full_unstemmed Hypertonic saline releases the attached small intestinal cystic fibrosis mucus
title_short Hypertonic saline releases the attached small intestinal cystic fibrosis mucus
title_sort hypertonic saline releases the attached small intestinal cystic fibrosis mucus
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4303949/
https://www.ncbi.nlm.nih.gov/pubmed/25311799
http://dx.doi.org/10.1111/1440-1681.12322
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