Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry

ARID1A is a known suppressor of tumour formation and the Human Protein Atlas antibody HPA005456 has been demonstrated in previous literature to stain tumour tissue by immunohistochemistry (IHC) in formalin-fixed paraffin embedded human tissue and human cell lines. This article details the validation...

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Detalles Bibliográficos
Autores principales: Howat, Will, Miller, Jodi, Gounaris, Ioannis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2015
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304311/
https://www.ncbi.nlm.nih.gov/pubmed/25653835
http://dx.doi.org/10.12688/f1000research.5514.2
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author Howat, Will
Miller, Jodi
Gounaris, Ioannis
author_facet Howat, Will
Miller, Jodi
Gounaris, Ioannis
author_sort Howat, Will
collection PubMed
description ARID1A is a known suppressor of tumour formation and the Human Protein Atlas antibody HPA005456 has been demonstrated in previous literature to stain tumour tissue by immunohistochemistry (IHC) in formalin-fixed paraffin embedded human tissue and human cell lines. This article details the validation of this antibody for immunohistochemistry of formalin-fixed paraffin embedded murine tissue using a Leica BondMax immunostainer. Using Western blot and IHC on murine wild-type and knockout tissue we have demonstrated that this antibody to ARID1A correctly stains murine tissue by immunohistochemistry.
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spelling pubmed-43043112015-02-03 Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry Howat, Will Miller, Jodi Gounaris, Ioannis F1000Res Research Note ARID1A is a known suppressor of tumour formation and the Human Protein Atlas antibody HPA005456 has been demonstrated in previous literature to stain tumour tissue by immunohistochemistry (IHC) in formalin-fixed paraffin embedded human tissue and human cell lines. This article details the validation of this antibody for immunohistochemistry of formalin-fixed paraffin embedded murine tissue using a Leica BondMax immunostainer. Using Western blot and IHC on murine wild-type and knockout tissue we have demonstrated that this antibody to ARID1A correctly stains murine tissue by immunohistochemistry. F1000Research 2015-01-06 /pmc/articles/PMC4304311/ /pubmed/25653835 http://dx.doi.org/10.12688/f1000research.5514.2 Text en Copyright: © 2015 Howat W et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/publicdomain/zero/1.0/ Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).
spellingShingle Research Note
Howat, Will
Miller, Jodi
Gounaris, Ioannis
Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry
title Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry
title_full Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry
title_fullStr Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry
title_full_unstemmed Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry
title_short Application of ARID1A to murine formalin-fixed paraffin embedded tissue using immunohistochemistry
title_sort application of arid1a to murine formalin-fixed paraffin embedded tissue using immunohistochemistry
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304311/
https://www.ncbi.nlm.nih.gov/pubmed/25653835
http://dx.doi.org/10.12688/f1000research.5514.2
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