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Direct Mutagenesis of Thousands of Genomic Targets Using Microarray-Derived Oligonucleotides

[Image: see text] Multiplex Automated Genome Engineering (MAGE) allows simultaneous mutagenesis of multiple target sites in bacterial genomes using short oligonucleotides. However, large-scale mutagenesis requires hundreds to thousands of unique oligos, which are costly to synthesize and impossible...

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Detalles Bibliográficos
Autores principales:	Bonde, Mads T., Kosuri, Sriram, Genee, Hans J., Sarup-Lytzen, Kira, Church, George M., Sommer, Morten O.A., Wang, Harris H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2014
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304438/ https://www.ncbi.nlm.nih.gov/pubmed/24856730 http://dx.doi.org/10.1021/sb5001565

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