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Field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load
BACKGROUND: Production of cassava (Manihot esculenta Crantz), a food security crop in sub-Saharan Africa, is threatened by the spread of cassava brown streak disease (CBSD) which manifests in part as a corky necrosis in the storage root. It is caused by either of two virus species, Cassava brown str...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304613/ https://www.ncbi.nlm.nih.gov/pubmed/25526680 http://dx.doi.org/10.1186/s12985-014-0216-x |
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author | Kaweesi, Tadeo Kawuki, Robert Kyaligonza, Vincent Baguma, Yona Tusiime, Geoffrey Ferguson, Morag E |
author_facet | Kaweesi, Tadeo Kawuki, Robert Kyaligonza, Vincent Baguma, Yona Tusiime, Geoffrey Ferguson, Morag E |
author_sort | Kaweesi, Tadeo |
collection | PubMed |
description | BACKGROUND: Production of cassava (Manihot esculenta Crantz), a food security crop in sub-Saharan Africa, is threatened by the spread of cassava brown streak disease (CBSD) which manifests in part as a corky necrosis in the storage root. It is caused by either of two virus species, Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), resulting in up to 100% yield loss in susceptible varieties. METHODS: This study characterized the response of 11 cassava varieties according to CBSD symptom expression and relative CBSV and UCBSV load in a field trial in Uganda. Relative viral load was measured using quantitative RT-PCR using COX as an internal housekeeping gene. RESULTS: A complex situation was revealed with indications of different resistance mechanisms that restrict virus accumulation and symptom expression. Four response categories were defined. Symptom expression was not always positively correlated with virus load. Substantially different levels of the virus species were found in many genotypes suggesting either resistance to one virus species or the other, or some form of interaction, antagonism or competition between virus species. CONCLUSIONS: A substantial amount of research still needs to be undertaken to fully understand the mechanism and genetic bases of resistance. This information will be useful in informing breeding strategies and restricting virus spread. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-014-0216-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4304613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43046132015-01-24 Field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load Kaweesi, Tadeo Kawuki, Robert Kyaligonza, Vincent Baguma, Yona Tusiime, Geoffrey Ferguson, Morag E Virol J Research BACKGROUND: Production of cassava (Manihot esculenta Crantz), a food security crop in sub-Saharan Africa, is threatened by the spread of cassava brown streak disease (CBSD) which manifests in part as a corky necrosis in the storage root. It is caused by either of two virus species, Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), resulting in up to 100% yield loss in susceptible varieties. METHODS: This study characterized the response of 11 cassava varieties according to CBSD symptom expression and relative CBSV and UCBSV load in a field trial in Uganda. Relative viral load was measured using quantitative RT-PCR using COX as an internal housekeeping gene. RESULTS: A complex situation was revealed with indications of different resistance mechanisms that restrict virus accumulation and symptom expression. Four response categories were defined. Symptom expression was not always positively correlated with virus load. Substantially different levels of the virus species were found in many genotypes suggesting either resistance to one virus species or the other, or some form of interaction, antagonism or competition between virus species. CONCLUSIONS: A substantial amount of research still needs to be undertaken to fully understand the mechanism and genetic bases of resistance. This information will be useful in informing breeding strategies and restricting virus spread. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-014-0216-x) contains supplementary material, which is available to authorized users. BioMed Central 2014-12-20 /pmc/articles/PMC4304613/ /pubmed/25526680 http://dx.doi.org/10.1186/s12985-014-0216-x Text en © Kaweesi et al.; licensee BioMed Central. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Kaweesi, Tadeo Kawuki, Robert Kyaligonza, Vincent Baguma, Yona Tusiime, Geoffrey Ferguson, Morag E Field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load |
title | Field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load |
title_full | Field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load |
title_fullStr | Field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load |
title_full_unstemmed | Field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load |
title_short | Field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load |
title_sort | field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304613/ https://www.ncbi.nlm.nih.gov/pubmed/25526680 http://dx.doi.org/10.1186/s12985-014-0216-x |
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