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High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues

An imaging platform based on broadband coherent anti-Stokes Raman scattering (BCARS) has been developed which provides an advantageous combination of speed, sensitivity and spectral breadth. The system utilizes a configuration of laser sources that probes the entire biologically-relevant Raman windo...

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Detalles Bibliográficos
Autores principales: Camp, Charles H., Lee, Young Jong, Heddleston, John M., Hartshorn, Christopher M., Hight Walker, Angela R., Rich, Jeremy N., Lathia, Justin D., Cicerone, Marcus T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304702/
https://www.ncbi.nlm.nih.gov/pubmed/25621002
http://dx.doi.org/10.1038/nphoton.2014.145
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author Camp, Charles H.
Lee, Young Jong
Heddleston, John M.
Hartshorn, Christopher M.
Hight Walker, Angela R.
Rich, Jeremy N.
Lathia, Justin D.
Cicerone, Marcus T.
author_facet Camp, Charles H.
Lee, Young Jong
Heddleston, John M.
Hartshorn, Christopher M.
Hight Walker, Angela R.
Rich, Jeremy N.
Lathia, Justin D.
Cicerone, Marcus T.
author_sort Camp, Charles H.
collection PubMed
description An imaging platform based on broadband coherent anti-Stokes Raman scattering (BCARS) has been developed which provides an advantageous combination of speed, sensitivity and spectral breadth. The system utilizes a configuration of laser sources that probes the entire biologically-relevant Raman window (500 cm(−1) to 3500 cm(−1)) with high resolution (< 10 cm(−1)). It strongly and efficiently stimulates Raman transitions within the typically weak “fingerprint” region using intrapulse 3-colour excitation, and utilizes the nonresonant background (NRB) to heterodyne amplify weak Raman signals. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumours and the surrounding healthy brain matter.
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spelling pubmed-43047022015-01-23 High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues Camp, Charles H. Lee, Young Jong Heddleston, John M. Hartshorn, Christopher M. Hight Walker, Angela R. Rich, Jeremy N. Lathia, Justin D. Cicerone, Marcus T. Nat Photonics Article An imaging platform based on broadband coherent anti-Stokes Raman scattering (BCARS) has been developed which provides an advantageous combination of speed, sensitivity and spectral breadth. The system utilizes a configuration of laser sources that probes the entire biologically-relevant Raman window (500 cm(−1) to 3500 cm(−1)) with high resolution (< 10 cm(−1)). It strongly and efficiently stimulates Raman transitions within the typically weak “fingerprint” region using intrapulse 3-colour excitation, and utilizes the nonresonant background (NRB) to heterodyne amplify weak Raman signals. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumours and the surrounding healthy brain matter. 2014 /pmc/articles/PMC4304702/ /pubmed/25621002 http://dx.doi.org/10.1038/nphoton.2014.145 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Camp, Charles H.
Lee, Young Jong
Heddleston, John M.
Hartshorn, Christopher M.
Hight Walker, Angela R.
Rich, Jeremy N.
Lathia, Justin D.
Cicerone, Marcus T.
High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues
title High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues
title_full High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues
title_fullStr High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues
title_full_unstemmed High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues
title_short High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues
title_sort high-speed coherent raman fingerprint imaging of biological tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304702/
https://www.ncbi.nlm.nih.gov/pubmed/25621002
http://dx.doi.org/10.1038/nphoton.2014.145
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