Cargando…
Experimental Study of Nasopharyngeal Carcinoma Radionuclide Imaging and Therapy Using Transferred Human Sodium/Iodide Symporter Gene
PURPOSE: The aim of this study was to design a method of radionuclide for imaging and therapy of nasopharyngeal carcinoma (NPC) using the transferred human sodium/iodide symporter (hNIS) gene. METHODS: A stable NPC cell line expressing hNIS was established (CNE-2-hNIS). After (131)I treatment, we de...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304840/ https://www.ncbi.nlm.nih.gov/pubmed/25615643 http://dx.doi.org/10.1371/journal.pone.0117053 |
_version_ | 1782354166449963008 |
---|---|
author | Zhong, Xing Shi, Changzheng Gong, Jian Guo, Bin Li, Mingzhu Xu, Hao |
author_facet | Zhong, Xing Shi, Changzheng Gong, Jian Guo, Bin Li, Mingzhu Xu, Hao |
author_sort | Zhong, Xing |
collection | PubMed |
description | PURPOSE: The aim of this study was to design a method of radionuclide for imaging and therapy of nasopharyngeal carcinoma (NPC) using the transferred human sodium/iodide symporter (hNIS) gene. METHODS: A stable NPC cell line expressing hNIS was established (CNE-2-hNIS). After (131)I treatment, we detected proliferation and apoptosis of NPC cells, both in vitro and vivo. In vivo, the radioactivity of different organs of nude mice was counted and (99m)Tc imaging using SPECT was performed. The apparent diffusion coefficient (ADC) value changes of tumor xenografts were observed by diffusion-weighted magnetic resonance imaging (DW-MRI) within 6–24 days of (131)I treatment. The correlation of ADC changes with apoptosis and proliferation was investigated. Post-treatment expression levels of P53, Bax, Bcl-2, Caspase-3, and Survivin proteins were detected by western blotting. RESULTS: (131)I uptake was higher in CNE-2-hNIS than in CNE-2 cells. The proliferation and apoptosis rate decreased and increased respectively both in vitro and vivo in the experimental group after (131)I treatment. The experimental group tumors accumulated (99m)Tc in vivo, leading to a good visualization by SPECT. DW-MRI showed that ADC values increased in the experimental group 6 days after treatment, while ADC values were positively and negatively correlated with the apoptotic and Ki-67 proliferation indices, respectively. After treatment, CNE-2-hNIS cells up-regulated the expression of P53 and Survivin proteins and activated Caspase-3, and down-regulated the expression of Bcl-2 proteins. CONCLUSIONS: The radionuclide imaging and therapy technique for NPC hNIS-transfected cell lines can provide a new therapy strategy for monitoring and treatment of NPC. |
format | Online Article Text |
id | pubmed-4304840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43048402015-01-30 Experimental Study of Nasopharyngeal Carcinoma Radionuclide Imaging and Therapy Using Transferred Human Sodium/Iodide Symporter Gene Zhong, Xing Shi, Changzheng Gong, Jian Guo, Bin Li, Mingzhu Xu, Hao PLoS One Research Article PURPOSE: The aim of this study was to design a method of radionuclide for imaging and therapy of nasopharyngeal carcinoma (NPC) using the transferred human sodium/iodide symporter (hNIS) gene. METHODS: A stable NPC cell line expressing hNIS was established (CNE-2-hNIS). After (131)I treatment, we detected proliferation and apoptosis of NPC cells, both in vitro and vivo. In vivo, the radioactivity of different organs of nude mice was counted and (99m)Tc imaging using SPECT was performed. The apparent diffusion coefficient (ADC) value changes of tumor xenografts were observed by diffusion-weighted magnetic resonance imaging (DW-MRI) within 6–24 days of (131)I treatment. The correlation of ADC changes with apoptosis and proliferation was investigated. Post-treatment expression levels of P53, Bax, Bcl-2, Caspase-3, and Survivin proteins were detected by western blotting. RESULTS: (131)I uptake was higher in CNE-2-hNIS than in CNE-2 cells. The proliferation and apoptosis rate decreased and increased respectively both in vitro and vivo in the experimental group after (131)I treatment. The experimental group tumors accumulated (99m)Tc in vivo, leading to a good visualization by SPECT. DW-MRI showed that ADC values increased in the experimental group 6 days after treatment, while ADC values were positively and negatively correlated with the apoptotic and Ki-67 proliferation indices, respectively. After treatment, CNE-2-hNIS cells up-regulated the expression of P53 and Survivin proteins and activated Caspase-3, and down-regulated the expression of Bcl-2 proteins. CONCLUSIONS: The radionuclide imaging and therapy technique for NPC hNIS-transfected cell lines can provide a new therapy strategy for monitoring and treatment of NPC. Public Library of Science 2015-01-23 /pmc/articles/PMC4304840/ /pubmed/25615643 http://dx.doi.org/10.1371/journal.pone.0117053 Text en © 2015 Zhong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhong, Xing Shi, Changzheng Gong, Jian Guo, Bin Li, Mingzhu Xu, Hao Experimental Study of Nasopharyngeal Carcinoma Radionuclide Imaging and Therapy Using Transferred Human Sodium/Iodide Symporter Gene |
title | Experimental Study of Nasopharyngeal Carcinoma Radionuclide Imaging and Therapy Using Transferred Human Sodium/Iodide Symporter Gene |
title_full | Experimental Study of Nasopharyngeal Carcinoma Radionuclide Imaging and Therapy Using Transferred Human Sodium/Iodide Symporter Gene |
title_fullStr | Experimental Study of Nasopharyngeal Carcinoma Radionuclide Imaging and Therapy Using Transferred Human Sodium/Iodide Symporter Gene |
title_full_unstemmed | Experimental Study of Nasopharyngeal Carcinoma Radionuclide Imaging and Therapy Using Transferred Human Sodium/Iodide Symporter Gene |
title_short | Experimental Study of Nasopharyngeal Carcinoma Radionuclide Imaging and Therapy Using Transferred Human Sodium/Iodide Symporter Gene |
title_sort | experimental study of nasopharyngeal carcinoma radionuclide imaging and therapy using transferred human sodium/iodide symporter gene |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304840/ https://www.ncbi.nlm.nih.gov/pubmed/25615643 http://dx.doi.org/10.1371/journal.pone.0117053 |
work_keys_str_mv | AT zhongxing experimentalstudyofnasopharyngealcarcinomaradionuclideimagingandtherapyusingtransferredhumansodiumiodidesymportergene AT shichangzheng experimentalstudyofnasopharyngealcarcinomaradionuclideimagingandtherapyusingtransferredhumansodiumiodidesymportergene AT gongjian experimentalstudyofnasopharyngealcarcinomaradionuclideimagingandtherapyusingtransferredhumansodiumiodidesymportergene AT guobin experimentalstudyofnasopharyngealcarcinomaradionuclideimagingandtherapyusingtransferredhumansodiumiodidesymportergene AT limingzhu experimentalstudyofnasopharyngealcarcinomaradionuclideimagingandtherapyusingtransferredhumansodiumiodidesymportergene AT xuhao experimentalstudyofnasopharyngealcarcinomaradionuclideimagingandtherapyusingtransferredhumansodiumiodidesymportergene |