Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus

The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locu...

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Autores principales: Sousa-Santos, Carla, Fonseca, Paulo J., Amorim, Maria Clara P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2015
Materias:
Animal Behavior
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304857/
https://www.ncbi.nlm.nih.gov/pubmed/25653909
http://dx.doi.org/10.7717/peerj.731
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author Sousa-Santos, Carla
Fonseca, Paulo J.
Amorim, Maria Clara P.
author_facet Sousa-Santos, Carla
Fonseca, Paulo J.
Amorim, Maria Clara P.
author_sort Sousa-Santos, Carla
collection PubMed
description The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy–Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species.
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spelling pubmed-43048572015-02-04 Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus Sousa-Santos, Carla Fonseca, Paulo J. Amorim, Maria Clara P. PeerJ Animal Behavior The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy–Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species. PeerJ Inc. 2015-01-20 /pmc/articles/PMC4304857/ /pubmed/25653909 http://dx.doi.org/10.7717/peerj.731 Text en © 2015 Sousa-Santos et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Animal Behavior
Sousa-Santos, Carla
Fonseca, Paulo J.
Amorim, Maria Clara P.
Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus
title Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus
title_full Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus
title_fullStr Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus
title_full_unstemmed Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus
title_short Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus
title_sort development and characterization of novel microsatellite loci for lusitanian toadfish, halobatrachus didactylus
topic Animal Behavior
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304857/
https://www.ncbi.nlm.nih.gov/pubmed/25653909
http://dx.doi.org/10.7717/peerj.731
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