Identification of Valid Reference Genes for the Normalization of RT-qPCR Expression Studies in Human Breast Cancer Cell Lines Treated with and without Transient Transfection

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a powerful technique for examining gene expression changes during tumorigenesis. Target gene expression is generally normalized by a stably expressed endogenous reference gene; however, reference gene expression may differ amo...

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Detalles Bibliográficos
Autores principales: Liu, Lin-Lin, Zhao, Hui, Ma, Teng-Fei, Ge, Fei, Chen, Ce-Shi, Zhang, Ya-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4305315/
https://www.ncbi.nlm.nih.gov/pubmed/25617865
http://dx.doi.org/10.1371/journal.pone.0117058

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