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Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes

Environmental exposure to electromagnetic fields is potentially carcinogenic. The radical pair mechanism is considered the most feasible mechanism of interaction between weak magnetic fields encountered in our environment and biochemical systems. Radicals are abundant in biology, both as free radica...

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Detalles Bibliográficos
Autores principales: Messiha, Hanan L., Wongnate, Thanyaporn, Chaiyen, Pimchai, Jones, Alex R., Scrutton, Nigel S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4305418/
https://www.ncbi.nlm.nih.gov/pubmed/25505136
http://dx.doi.org/10.1098/rsif.2014.1155
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author Messiha, Hanan L.
Wongnate, Thanyaporn
Chaiyen, Pimchai
Jones, Alex R.
Scrutton, Nigel S.
author_facet Messiha, Hanan L.
Wongnate, Thanyaporn
Chaiyen, Pimchai
Jones, Alex R.
Scrutton, Nigel S.
author_sort Messiha, Hanan L.
collection PubMed
description Environmental exposure to electromagnetic fields is potentially carcinogenic. The radical pair mechanism is considered the most feasible mechanism of interaction between weak magnetic fields encountered in our environment and biochemical systems. Radicals are abundant in biology, both as free radicals and reaction intermediates in enzyme mechanisms. The catalytic cycles of some flavin-dependent enzymes are either known or potentially involve radical pairs. Here, we have investigated the magnetic field sensitivity of a number of flavoenzymes with important cellular roles. We also investigated the magnetic field sensitivity of a model system involving stepwise reduction of a flavin analogue by a nicotinamide analogue—a reaction known to proceed via a radical pair. Under the experimental conditions used, magnetic field sensitivity was not observed in the reaction kinetics from stopped-flow measurements in any of the systems studied. Although widely implicated in radical pair chemistry, we conclude that thermally driven, flavoenzyme-catalysed reactions are unlikely to be influenced by exposure to external magnetic fields.
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spelling pubmed-43054182015-02-06 Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes Messiha, Hanan L. Wongnate, Thanyaporn Chaiyen, Pimchai Jones, Alex R. Scrutton, Nigel S. J R Soc Interface Research Articles Environmental exposure to electromagnetic fields is potentially carcinogenic. The radical pair mechanism is considered the most feasible mechanism of interaction between weak magnetic fields encountered in our environment and biochemical systems. Radicals are abundant in biology, both as free radicals and reaction intermediates in enzyme mechanisms. The catalytic cycles of some flavin-dependent enzymes are either known or potentially involve radical pairs. Here, we have investigated the magnetic field sensitivity of a number of flavoenzymes with important cellular roles. We also investigated the magnetic field sensitivity of a model system involving stepwise reduction of a flavin analogue by a nicotinamide analogue—a reaction known to proceed via a radical pair. Under the experimental conditions used, magnetic field sensitivity was not observed in the reaction kinetics from stopped-flow measurements in any of the systems studied. Although widely implicated in radical pair chemistry, we conclude that thermally driven, flavoenzyme-catalysed reactions are unlikely to be influenced by exposure to external magnetic fields. The Royal Society 2015-02-06 /pmc/articles/PMC4305418/ /pubmed/25505136 http://dx.doi.org/10.1098/rsif.2014.1155 Text en http://creativecommons.org/licenses/by/4.0/ © 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research Articles
Messiha, Hanan L.
Wongnate, Thanyaporn
Chaiyen, Pimchai
Jones, Alex R.
Scrutton, Nigel S.
Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes
title Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes
title_full Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes
title_fullStr Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes
title_full_unstemmed Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes
title_short Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes
title_sort magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4305418/
https://www.ncbi.nlm.nih.gov/pubmed/25505136
http://dx.doi.org/10.1098/rsif.2014.1155
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