Cargando…
iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona
BACKGROUND: Validation of digital whole slide images is crucial to ensure that diagnostic performance is at least equivalent to that of glass slides and light microscopy. The College of American Pathologists Pathology and Laboratory Quality Center recently developed recommendations for internal digi...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4305970/ https://www.ncbi.nlm.nih.gov/pubmed/25565219 http://dx.doi.org/10.1186/1746-1596-9-S1-S12 |
_version_ | 1782354253187121152 |
---|---|
author | Brunelli, Matteo Beccari, Serena Colombari, Romano Gobbo, Stefano Giobelli, Luca Pellegrini, Andrea Chilosi, Marco Lunardi, Maria Martignoni, Guido Scarpa, Aldo Eccher, Albino |
author_facet | Brunelli, Matteo Beccari, Serena Colombari, Romano Gobbo, Stefano Giobelli, Luca Pellegrini, Andrea Chilosi, Marco Lunardi, Maria Martignoni, Guido Scarpa, Aldo Eccher, Albino |
author_sort | Brunelli, Matteo |
collection | PubMed |
description | BACKGROUND: Validation of digital whole slide images is crucial to ensure that diagnostic performance is at least equivalent to that of glass slides and light microscopy. The College of American Pathologists Pathology and Laboratory Quality Center recently developed recommendations for internal digital pathology system validation. Following these guidelines we sought to validate the performance of a digital approach for routine diagnosis by using an iPad and digital control widescreen-assisted workstation through a pilot study. METHODS: From January 2014, 61 histopathological slides were scanned by ScanScope Digital Slides Scanner (Aperio, Vista, CA). Two independent pathologists performed diagnosis on virtual slides in front of a widescreen by using two computer devices (ImageScope viewing software) located to different Health Institutions (AOUI Verona) connected by local network and a remote image server using an iPad tablet (Aperio, Vista, CA), after uploading the Citrix receiver for iPad. Quality indicators related to image characters and work-flow of the e-health cockpit enterprise system were scored based on subjective (high vs poor) perception. The images were re-evaluated two weeks apart. RESULTS: The whole glass slides encountered 10 liver: hepatocarcinoma, 10 renal carcinoma, 10 gastric carcinoma and 10 prostate biopsies: adenocarcinoma, 5 excisional skin biopsies: melanoma, 5 lymph-nodes: lymphoma. 6 immuno- and 5 special stains were available for intra- and internet remote viewing. Scan times averaged two minutes and 54 seconds per slide (standard deviation 2 minutes 34 seconds). Megabytes ranged from 256 to 680 (mean 390) per slide storage. Reliance on glass slide, image quality (resolution and color fidelity), slide navigation time, simultaneous viewers in geographically remote locations were considered of high performance score. Side by side comparisons between diagnosis performed on tissue glass slides versus widescreen were excellent showing an almost perfect concordance (0.81, kappa index). CONCLUSIONS: We validated our institutional digital pathology system for routine diagnostic facing with whole slide images in a cockpit enterprise digital system or iPad tablet. Computer widescreens are better for diagnosing scanned glass slide that iPad. For urgent requests, iPad may be used. Legal aspects have to be soon faced with to permit the clinical use of this technology in a manner that does not compromise patient care. |
format | Online Article Text |
id | pubmed-4305970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43059702015-02-12 iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona Brunelli, Matteo Beccari, Serena Colombari, Romano Gobbo, Stefano Giobelli, Luca Pellegrini, Andrea Chilosi, Marco Lunardi, Maria Martignoni, Guido Scarpa, Aldo Eccher, Albino Diagn Pathol Proceedings BACKGROUND: Validation of digital whole slide images is crucial to ensure that diagnostic performance is at least equivalent to that of glass slides and light microscopy. The College of American Pathologists Pathology and Laboratory Quality Center recently developed recommendations for internal digital pathology system validation. Following these guidelines we sought to validate the performance of a digital approach for routine diagnosis by using an iPad and digital control widescreen-assisted workstation through a pilot study. METHODS: From January 2014, 61 histopathological slides were scanned by ScanScope Digital Slides Scanner (Aperio, Vista, CA). Two independent pathologists performed diagnosis on virtual slides in front of a widescreen by using two computer devices (ImageScope viewing software) located to different Health Institutions (AOUI Verona) connected by local network and a remote image server using an iPad tablet (Aperio, Vista, CA), after uploading the Citrix receiver for iPad. Quality indicators related to image characters and work-flow of the e-health cockpit enterprise system were scored based on subjective (high vs poor) perception. The images were re-evaluated two weeks apart. RESULTS: The whole glass slides encountered 10 liver: hepatocarcinoma, 10 renal carcinoma, 10 gastric carcinoma and 10 prostate biopsies: adenocarcinoma, 5 excisional skin biopsies: melanoma, 5 lymph-nodes: lymphoma. 6 immuno- and 5 special stains were available for intra- and internet remote viewing. Scan times averaged two minutes and 54 seconds per slide (standard deviation 2 minutes 34 seconds). Megabytes ranged from 256 to 680 (mean 390) per slide storage. Reliance on glass slide, image quality (resolution and color fidelity), slide navigation time, simultaneous viewers in geographically remote locations were considered of high performance score. Side by side comparisons between diagnosis performed on tissue glass slides versus widescreen were excellent showing an almost perfect concordance (0.81, kappa index). CONCLUSIONS: We validated our institutional digital pathology system for routine diagnostic facing with whole slide images in a cockpit enterprise digital system or iPad tablet. Computer widescreens are better for diagnosing scanned glass slide that iPad. For urgent requests, iPad may be used. Legal aspects have to be soon faced with to permit the clinical use of this technology in a manner that does not compromise patient care. BioMed Central 2014-12-19 /pmc/articles/PMC4305970/ /pubmed/25565219 http://dx.doi.org/10.1186/1746-1596-9-S1-S12 Text en Copyright © 2014 Brunelli et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Proceedings Brunelli, Matteo Beccari, Serena Colombari, Romano Gobbo, Stefano Giobelli, Luca Pellegrini, Andrea Chilosi, Marco Lunardi, Maria Martignoni, Guido Scarpa, Aldo Eccher, Albino iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona |
title | iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona |
title_full | iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona |
title_fullStr | iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona |
title_full_unstemmed | iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona |
title_short | iPathology cockpit diagnostic station: validation according to College of American Pathologists Pathology and Laboratory Quality Center recommendation at the Hospital Trust and University of Verona |
title_sort | ipathology cockpit diagnostic station: validation according to college of american pathologists pathology and laboratory quality center recommendation at the hospital trust and university of verona |
topic | Proceedings |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4305970/ https://www.ncbi.nlm.nih.gov/pubmed/25565219 http://dx.doi.org/10.1186/1746-1596-9-S1-S12 |
work_keys_str_mv | AT brunellimatteo ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona AT beccariserena ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona AT colombariromano ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona AT gobbostefano ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona AT giobelliluca ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona AT pellegriniandrea ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona AT chilosimarco ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona AT lunardimaria ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona AT martignoniguido ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona AT scarpaaldo ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona AT eccheralbino ipathologycockpitdiagnosticstationvalidationaccordingtocollegeofamericanpathologistspathologyandlaboratoryqualitycenterrecommendationatthehospitaltrustanduniversityofverona |