New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin

We describe a new method for determining the concentration of total Ca in whole skeletal muscle samples ([Ca(T)](WM) in units of mmoles/kg wet weight) using the Ca-dependent UV absorbance spectra of the Ca chelator BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid). Muscle tissue was h...

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Detalles Bibliográficos
Autores principales: Lamboley, Cédric R.H., Kake Guena, Sandrine A., Touré, Fatou, Hébert, Camille, Yaddaden, Louiza, Nadeau, Stephanie, Bouchard, Patrice, Wei-LaPierre, Lan, Lainé, Jean, Rousseau, Eric C., Frenette, Jérôme, Protasi, Feliciano, Dirksen, Robert T., Pape, Paul C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Methods and Approaches
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306712/
https://www.ncbi.nlm.nih.gov/pubmed/25624449
http://dx.doi.org/10.1085/jgp.201411250

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