Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts

We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in...

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Autores principales: Yamayoshi, Seiya, Fukuyama, Satoshi, Yamada, Shinya, Zhao, Dongming, Murakami, Shin, Uraki, Ryuta, Watanabe, Tokiko, Tomita, Yuriko, Neumann, Gabriele, Kawaoka, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306964/
https://www.ncbi.nlm.nih.gov/pubmed/25623817
http://dx.doi.org/10.1038/srep08039
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author Yamayoshi, Seiya
Fukuyama, Satoshi
Yamada, Shinya
Zhao, Dongming
Murakami, Shin
Uraki, Ryuta
Watanabe, Tokiko
Tomita, Yuriko
Neumann, Gabriele
Kawaoka, Yoshihiro
author_facet Yamayoshi, Seiya
Fukuyama, Satoshi
Yamada, Shinya
Zhao, Dongming
Murakami, Shin
Uraki, Ryuta
Watanabe, Tokiko
Tomita, Yuriko
Neumann, Gabriele
Kawaoka, Yoshihiro
author_sort Yamayoshi, Seiya
collection PubMed
description We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in mammalian and avian cells, and pathogenicity in mice. We found that lysine at position 627 and asparagine at position 701 in PB2 are essential for mammalian adaptation of A(H7N9) viruses.
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spelling pubmed-43069642015-02-06 Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts Yamayoshi, Seiya Fukuyama, Satoshi Yamada, Shinya Zhao, Dongming Murakami, Shin Uraki, Ryuta Watanabe, Tokiko Tomita, Yuriko Neumann, Gabriele Kawaoka, Yoshihiro Sci Rep Article We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in mammalian and avian cells, and pathogenicity in mice. We found that lysine at position 627 and asparagine at position 701 in PB2 are essential for mammalian adaptation of A(H7N9) viruses. Nature Publishing Group 2015-01-27 /pmc/articles/PMC4306964/ /pubmed/25623817 http://dx.doi.org/10.1038/srep08039 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Yamayoshi, Seiya
Fukuyama, Satoshi
Yamada, Shinya
Zhao, Dongming
Murakami, Shin
Uraki, Ryuta
Watanabe, Tokiko
Tomita, Yuriko
Neumann, Gabriele
Kawaoka, Yoshihiro
Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts
title Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts
title_full Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts
title_fullStr Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts
title_full_unstemmed Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts
title_short Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts
title_sort amino acids substitutions in the pb2 protein of h7n9 influenza a viruses are important for virulence in mammalian hosts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306964/
https://www.ncbi.nlm.nih.gov/pubmed/25623817
http://dx.doi.org/10.1038/srep08039
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