Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma

The cAMP-regulated phosphoprotein 19 (ARPP-19) plays a key role in cell mitotic G2/M transition. Expression of ARPP-19 was increased in human hepatocellular carcinoma (HCC) compared to adjacent non-tumorous liver tissues in 36 paired liver samples, and the level of ARPP-19 in HCC tissues was positiv...

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Autores principales: Song, Haiyan, Pan, Jielu, Liu, Yang, Wen, Hongzhu, Wang, Lei, Cui, Jiefeng, Liu, Yinkun, Hu, Bing, Yao, Zemin, Ji, Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307242/
https://www.ncbi.nlm.nih.gov/pubmed/25547487
http://dx.doi.org/10.3390/ijms16010178
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author Song, Haiyan
Pan, Jielu
Liu, Yang
Wen, Hongzhu
Wang, Lei
Cui, Jiefeng
Liu, Yinkun
Hu, Bing
Yao, Zemin
Ji, Guang
author_facet Song, Haiyan
Pan, Jielu
Liu, Yang
Wen, Hongzhu
Wang, Lei
Cui, Jiefeng
Liu, Yinkun
Hu, Bing
Yao, Zemin
Ji, Guang
author_sort Song, Haiyan
collection PubMed
description The cAMP-regulated phosphoprotein 19 (ARPP-19) plays a key role in cell mitotic G2/M transition. Expression of ARPP-19 was increased in human hepatocellular carcinoma (HCC) compared to adjacent non-tumorous liver tissues in 36 paired liver samples, and the level of ARPP-19 in HCC tissues was positively correlated with the tumor size. To determine the interrelationship between ARPP-19 expression and HCC, we silenced ARPP-19 expression in the human hepatocarcinoma HepG2 and SMMC-7721 cells using lentivirus encoding ARPP-19 siRNA. HepG2 and SMMC-7721 cells with ARPP-19 knockdown displayed lowered cell growth rate, retarded colony formation and increased arrest at the G2/M phase transition. Silencing ARPP-19 in HCC cells resulted in decreased protein levels of phospho-(Ser) CDKs substrates and increased levels of inactivated cyclin division cycle 2 (Cdc2). Therefore, ARPP-19 may play a role in HCC pathogenesis through regulating cell proliferation.
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spelling pubmed-43072422015-02-02 Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma Song, Haiyan Pan, Jielu Liu, Yang Wen, Hongzhu Wang, Lei Cui, Jiefeng Liu, Yinkun Hu, Bing Yao, Zemin Ji, Guang Int J Mol Sci Article The cAMP-regulated phosphoprotein 19 (ARPP-19) plays a key role in cell mitotic G2/M transition. Expression of ARPP-19 was increased in human hepatocellular carcinoma (HCC) compared to adjacent non-tumorous liver tissues in 36 paired liver samples, and the level of ARPP-19 in HCC tissues was positively correlated with the tumor size. To determine the interrelationship between ARPP-19 expression and HCC, we silenced ARPP-19 expression in the human hepatocarcinoma HepG2 and SMMC-7721 cells using lentivirus encoding ARPP-19 siRNA. HepG2 and SMMC-7721 cells with ARPP-19 knockdown displayed lowered cell growth rate, retarded colony formation and increased arrest at the G2/M phase transition. Silencing ARPP-19 in HCC cells resulted in decreased protein levels of phospho-(Ser) CDKs substrates and increased levels of inactivated cyclin division cycle 2 (Cdc2). Therefore, ARPP-19 may play a role in HCC pathogenesis through regulating cell proliferation. MDPI 2014-12-24 /pmc/articles/PMC4307242/ /pubmed/25547487 http://dx.doi.org/10.3390/ijms16010178 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Haiyan
Pan, Jielu
Liu, Yang
Wen, Hongzhu
Wang, Lei
Cui, Jiefeng
Liu, Yinkun
Hu, Bing
Yao, Zemin
Ji, Guang
Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title_full Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title_fullStr Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title_full_unstemmed Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title_short Increased ARPP-19 Expression Is Associated with Hepatocellular Carcinoma
title_sort increased arpp-19 expression is associated with hepatocellular carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307242/
https://www.ncbi.nlm.nih.gov/pubmed/25547487
http://dx.doi.org/10.3390/ijms16010178
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