Cargando…

Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma

This study aims to elucidate the level of DNA methylation in urothelial carcinomas (UCs) using 5-methylcytosine (5-MeC) immunohistochemistry (IHC). We examined the relationship among 5-MeC levels, DNA (cytosine-5)-methyltransferase 1 (DNMT1) immunostaining levels, and clinicopathologic features. Tis...

Descripción completa

Detalles Bibliográficos
Autores principales: Chung, Chi-Jung, Chang, Chao-Hsiang, Chuu, Chih-Pin, Yang, Chi-Rei, Chang, Yi-Huei, Huang, Chi-Ping, Chen, Wen-Chi, Chung, Mu-Chi, Chang, Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307268/
https://www.ncbi.nlm.nih.gov/pubmed/25561224
http://dx.doi.org/10.3390/ijms16010677
_version_ 1782354434397831168
author Chung, Chi-Jung
Chang, Chao-Hsiang
Chuu, Chih-Pin
Yang, Chi-Rei
Chang, Yi-Huei
Huang, Chi-Ping
Chen, Wen-Chi
Chung, Mu-Chi
Chang, Han
author_facet Chung, Chi-Jung
Chang, Chao-Hsiang
Chuu, Chih-Pin
Yang, Chi-Rei
Chang, Yi-Huei
Huang, Chi-Ping
Chen, Wen-Chi
Chung, Mu-Chi
Chang, Han
author_sort Chung, Chi-Jung
collection PubMed
description This study aims to elucidate the level of DNA methylation in urothelial carcinomas (UCs) using 5-methylcytosine (5-MeC) immunohistochemistry (IHC). We examined the relationship among 5-MeC levels, DNA (cytosine-5)-methyltransferase 1 (DNMT1) immunostaining levels, and clinicopathologic features. Tissue samples included 23 normal urothelia and 150 urothelial neoplasia, which comprised 40 non-invasive and 110 invasive UCs. The levels of 5-MeC and DNMT1 were assessed based on their immunoreactivities and then divided into low and high levels. In addition, we collected information on clinical variables, pathologic features, and recurrent status from patient questionnaires and medical records. Chi-square test and multivariate logistic regression model were used for analyses. Results showed that 5-MeC levels were positively associated with DNMT1 levels in UC (p = 0.0288). Both 5-MeC and DNMT1 were low in approximately 50% (76/150) of UC. The percentage of low 5-MeC levels was higher in invasive UC (65/110; 59%) than in normal urothelia (2/23; 13%) and non-invasive UC (18/40; 45%). Clinical factors were independently associated with low 5-MeC levels after adjusting for age and sex, including cancer stages II–IV, presence of UC in situ, and marked inflammation. Low 5-MeC levels in stage I invasive UC were not significantly different from those of non-invasive tumors (p = 0.8478). Low DNMT1 levels were only associated with UC with squamous differentiation (p = 0.0365). Neither 5-MeC nor DNMT1 levels were associated with UC recurrence. In conclusion, a low 5-MeC level could predict the progression of UC invasion into muscle.
format Online
Article
Text
id pubmed-4307268
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-43072682015-02-02 Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma Chung, Chi-Jung Chang, Chao-Hsiang Chuu, Chih-Pin Yang, Chi-Rei Chang, Yi-Huei Huang, Chi-Ping Chen, Wen-Chi Chung, Mu-Chi Chang, Han Int J Mol Sci Article This study aims to elucidate the level of DNA methylation in urothelial carcinomas (UCs) using 5-methylcytosine (5-MeC) immunohistochemistry (IHC). We examined the relationship among 5-MeC levels, DNA (cytosine-5)-methyltransferase 1 (DNMT1) immunostaining levels, and clinicopathologic features. Tissue samples included 23 normal urothelia and 150 urothelial neoplasia, which comprised 40 non-invasive and 110 invasive UCs. The levels of 5-MeC and DNMT1 were assessed based on their immunoreactivities and then divided into low and high levels. In addition, we collected information on clinical variables, pathologic features, and recurrent status from patient questionnaires and medical records. Chi-square test and multivariate logistic regression model were used for analyses. Results showed that 5-MeC levels were positively associated with DNMT1 levels in UC (p = 0.0288). Both 5-MeC and DNMT1 were low in approximately 50% (76/150) of UC. The percentage of low 5-MeC levels was higher in invasive UC (65/110; 59%) than in normal urothelia (2/23; 13%) and non-invasive UC (18/40; 45%). Clinical factors were independently associated with low 5-MeC levels after adjusting for age and sex, including cancer stages II–IV, presence of UC in situ, and marked inflammation. Low 5-MeC levels in stage I invasive UC were not significantly different from those of non-invasive tumors (p = 0.8478). Low DNMT1 levels were only associated with UC with squamous differentiation (p = 0.0365). Neither 5-MeC nor DNMT1 levels were associated with UC recurrence. In conclusion, a low 5-MeC level could predict the progression of UC invasion into muscle. MDPI 2014-12-30 /pmc/articles/PMC4307268/ /pubmed/25561224 http://dx.doi.org/10.3390/ijms16010677 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chung, Chi-Jung
Chang, Chao-Hsiang
Chuu, Chih-Pin
Yang, Chi-Rei
Chang, Yi-Huei
Huang, Chi-Ping
Chen, Wen-Chi
Chung, Mu-Chi
Chang, Han
Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma
title Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma
title_full Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma
title_fullStr Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma
title_full_unstemmed Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma
title_short Reduced 5-Methylcytosine Level as a Potential Progression Predictor in Patients with T1 or Non-Invasive Urothelial Carcinoma
title_sort reduced 5-methylcytosine level as a potential progression predictor in patients with t1 or non-invasive urothelial carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307268/
https://www.ncbi.nlm.nih.gov/pubmed/25561224
http://dx.doi.org/10.3390/ijms16010677
work_keys_str_mv AT chungchijung reduced5methylcytosinelevelasapotentialprogressionpredictorinpatientswitht1ornoninvasiveurothelialcarcinoma
AT changchaohsiang reduced5methylcytosinelevelasapotentialprogressionpredictorinpatientswitht1ornoninvasiveurothelialcarcinoma
AT chuuchihpin reduced5methylcytosinelevelasapotentialprogressionpredictorinpatientswitht1ornoninvasiveurothelialcarcinoma
AT yangchirei reduced5methylcytosinelevelasapotentialprogressionpredictorinpatientswitht1ornoninvasiveurothelialcarcinoma
AT changyihuei reduced5methylcytosinelevelasapotentialprogressionpredictorinpatientswitht1ornoninvasiveurothelialcarcinoma
AT huangchiping reduced5methylcytosinelevelasapotentialprogressionpredictorinpatientswitht1ornoninvasiveurothelialcarcinoma
AT chenwenchi reduced5methylcytosinelevelasapotentialprogressionpredictorinpatientswitht1ornoninvasiveurothelialcarcinoma
AT chungmuchi reduced5methylcytosinelevelasapotentialprogressionpredictorinpatientswitht1ornoninvasiveurothelialcarcinoma
AT changhan reduced5methylcytosinelevelasapotentialprogressionpredictorinpatientswitht1ornoninvasiveurothelialcarcinoma