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Ligand-Induced Dynamics of Neurotrophin Receptors Investigated by Single-Molecule Imaging Approaches

Neurotrophins are secreted proteins that regulate neuronal development and survival, as well as maintenance and plasticity of the adult nervous system. The biological activity of neurotrophins stems from their binding to two membrane receptor types, the tropomyosin receptor kinase and the p75 neurot...

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Autores principales: Marchetti, Laura, Luin, Stefano, Bonsignore, Fulvio, de Nadai, Teresa, Beltram, Fabio, Cattaneo, Antonino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307343/
https://www.ncbi.nlm.nih.gov/pubmed/25603178
http://dx.doi.org/10.3390/ijms16011949
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author Marchetti, Laura
Luin, Stefano
Bonsignore, Fulvio
de Nadai, Teresa
Beltram, Fabio
Cattaneo, Antonino
author_facet Marchetti, Laura
Luin, Stefano
Bonsignore, Fulvio
de Nadai, Teresa
Beltram, Fabio
Cattaneo, Antonino
author_sort Marchetti, Laura
collection PubMed
description Neurotrophins are secreted proteins that regulate neuronal development and survival, as well as maintenance and plasticity of the adult nervous system. The biological activity of neurotrophins stems from their binding to two membrane receptor types, the tropomyosin receptor kinase and the p75 neurotrophin receptors (NRs). The intracellular signalling cascades thereby activated have been extensively investigated. Nevertheless, a comprehensive description of the ligand-induced nanoscale details of NRs dynamics and interactions spanning from the initial lateral movements triggered at the plasma membrane to the internalization and transport processes is still missing. Recent advances in high spatio-temporal resolution imaging techniques have yielded new insight on the dynamics of NRs upon ligand binding. Here we discuss requirements, potential and practical implementation of these novel approaches for the study of neurotrophin trafficking and signalling, in the framework of current knowledge available also for other ligand-receptor systems. We shall especially highlight the correlation between the receptor dynamics activated by different neurotrophins and the respective signalling outcome, as recently revealed by single-molecule tracking of NRs in living neuronal cells.
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spelling pubmed-43073432015-02-02 Ligand-Induced Dynamics of Neurotrophin Receptors Investigated by Single-Molecule Imaging Approaches Marchetti, Laura Luin, Stefano Bonsignore, Fulvio de Nadai, Teresa Beltram, Fabio Cattaneo, Antonino Int J Mol Sci Review Neurotrophins are secreted proteins that regulate neuronal development and survival, as well as maintenance and plasticity of the adult nervous system. The biological activity of neurotrophins stems from their binding to two membrane receptor types, the tropomyosin receptor kinase and the p75 neurotrophin receptors (NRs). The intracellular signalling cascades thereby activated have been extensively investigated. Nevertheless, a comprehensive description of the ligand-induced nanoscale details of NRs dynamics and interactions spanning from the initial lateral movements triggered at the plasma membrane to the internalization and transport processes is still missing. Recent advances in high spatio-temporal resolution imaging techniques have yielded new insight on the dynamics of NRs upon ligand binding. Here we discuss requirements, potential and practical implementation of these novel approaches for the study of neurotrophin trafficking and signalling, in the framework of current knowledge available also for other ligand-receptor systems. We shall especially highlight the correlation between the receptor dynamics activated by different neurotrophins and the respective signalling outcome, as recently revealed by single-molecule tracking of NRs in living neuronal cells. MDPI 2015-01-16 /pmc/articles/PMC4307343/ /pubmed/25603178 http://dx.doi.org/10.3390/ijms16011949 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Marchetti, Laura
Luin, Stefano
Bonsignore, Fulvio
de Nadai, Teresa
Beltram, Fabio
Cattaneo, Antonino
Ligand-Induced Dynamics of Neurotrophin Receptors Investigated by Single-Molecule Imaging Approaches
title Ligand-Induced Dynamics of Neurotrophin Receptors Investigated by Single-Molecule Imaging Approaches
title_full Ligand-Induced Dynamics of Neurotrophin Receptors Investigated by Single-Molecule Imaging Approaches
title_fullStr Ligand-Induced Dynamics of Neurotrophin Receptors Investigated by Single-Molecule Imaging Approaches
title_full_unstemmed Ligand-Induced Dynamics of Neurotrophin Receptors Investigated by Single-Molecule Imaging Approaches
title_short Ligand-Induced Dynamics of Neurotrophin Receptors Investigated by Single-Molecule Imaging Approaches
title_sort ligand-induced dynamics of neurotrophin receptors investigated by single-molecule imaging approaches
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307343/
https://www.ncbi.nlm.nih.gov/pubmed/25603178
http://dx.doi.org/10.3390/ijms16011949
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