Cargando…

Insecticide-Mediated Up-Regulation of Cytochrome P450 Genes in the Red Flour Beetle (Tribolium castaneum)

Some cytochrome P450 (CYP) genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated...

Descripción completa

Detalles Bibliográficos
Autores principales: Liang, Xiao, Xiao, Da, He, Yanping, Yao, Jianxiu, Zhu, Guonian, Zhu, Kun Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307350/
https://www.ncbi.nlm.nih.gov/pubmed/25607733
http://dx.doi.org/10.3390/ijms16012078
_version_ 1782354454032416768
author Liang, Xiao
Xiao, Da
He, Yanping
Yao, Jianxiu
Zhu, Guonian
Zhu, Kun Yan
author_facet Liang, Xiao
Xiao, Da
He, Yanping
Yao, Jianxiu
Zhu, Guonian
Zhu, Kun Yan
author_sort Liang, Xiao
collection PubMed
description Some cytochrome P450 (CYP) genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC(20) for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC(20) in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.
format Online
Article
Text
id pubmed-4307350
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-43073502015-02-02 Insecticide-Mediated Up-Regulation of Cytochrome P450 Genes in the Red Flour Beetle (Tribolium castaneum) Liang, Xiao Xiao, Da He, Yanping Yao, Jianxiu Zhu, Guonian Zhu, Kun Yan Int J Mol Sci Article Some cytochrome P450 (CYP) genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC(20) for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC(20) in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification. MDPI 2015-01-19 /pmc/articles/PMC4307350/ /pubmed/25607733 http://dx.doi.org/10.3390/ijms16012078 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liang, Xiao
Xiao, Da
He, Yanping
Yao, Jianxiu
Zhu, Guonian
Zhu, Kun Yan
Insecticide-Mediated Up-Regulation of Cytochrome P450 Genes in the Red Flour Beetle (Tribolium castaneum)
title Insecticide-Mediated Up-Regulation of Cytochrome P450 Genes in the Red Flour Beetle (Tribolium castaneum)
title_full Insecticide-Mediated Up-Regulation of Cytochrome P450 Genes in the Red Flour Beetle (Tribolium castaneum)
title_fullStr Insecticide-Mediated Up-Regulation of Cytochrome P450 Genes in the Red Flour Beetle (Tribolium castaneum)
title_full_unstemmed Insecticide-Mediated Up-Regulation of Cytochrome P450 Genes in the Red Flour Beetle (Tribolium castaneum)
title_short Insecticide-Mediated Up-Regulation of Cytochrome P450 Genes in the Red Flour Beetle (Tribolium castaneum)
title_sort insecticide-mediated up-regulation of cytochrome p450 genes in the red flour beetle (tribolium castaneum)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307350/
https://www.ncbi.nlm.nih.gov/pubmed/25607733
http://dx.doi.org/10.3390/ijms16012078
work_keys_str_mv AT liangxiao insecticidemediatedupregulationofcytochromep450genesintheredflourbeetletriboliumcastaneum
AT xiaoda insecticidemediatedupregulationofcytochromep450genesintheredflourbeetletriboliumcastaneum
AT heyanping insecticidemediatedupregulationofcytochromep450genesintheredflourbeetletriboliumcastaneum
AT yaojianxiu insecticidemediatedupregulationofcytochromep450genesintheredflourbeetletriboliumcastaneum
AT zhuguonian insecticidemediatedupregulationofcytochromep450genesintheredflourbeetletriboliumcastaneum
AT zhukunyan insecticidemediatedupregulationofcytochromep450genesintheredflourbeetletriboliumcastaneum