Cargando…

Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits

BACKGROUND: Animal acariasis is one of the important veterinary skin diseases. Chemical drugs have been widely used to treat and control this kind of disease. But many chemicals control could increase resistance in target species, toxicity and environmental hazards. We found that the 9-oxo-10, 11-de...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Yang, Liao, Fei, Hu, Yanchun, Luo, Biao, He, Yajun, Mo, Quan, Zuo, Zhicai, Ren, Zhihua, Deng, Junliang, Wei, Yahui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308076/
https://www.ncbi.nlm.nih.gov/pubmed/25527276
http://dx.doi.org/10.1186/s12917-014-0306-4
_version_ 1782354544435396608
author Hu, Yang
Liao, Fei
Hu, Yanchun
Luo, Biao
He, Yajun
Mo, Quan
Zuo, Zhicai
Ren, Zhihua
Deng, Junliang
Wei, Yahui
author_facet Hu, Yang
Liao, Fei
Hu, Yanchun
Luo, Biao
He, Yajun
Mo, Quan
Zuo, Zhicai
Ren, Zhihua
Deng, Junliang
Wei, Yahui
author_sort Hu, Yang
collection PubMed
description BACKGROUND: Animal acariasis is one of the important veterinary skin diseases. Chemical drugs have been widely used to treat and control this kind of disease. But many chemicals control could increase resistance in target species, toxicity and environmental hazards. We found that the 9-oxo-10, 11-dehydroageraphorone (euptox A) extracted from E. adenophorum has strong toxicity against P. cuniculi in vitro, but the in vivo acaricidal actions of euptox A have yet to be investigated. RESULTS: A 14-day experiment was performed using rabbits that were naturally infested with P. cuniculi on a farm. Rabbits were randomly divided into five groups; animals in groups A, B and C were treated in each ear topically with 4.0 ml of 2.0 and 1.0 g/L (w/v) euptox A, respectively. Animals in groups D and E were treated with ivermectin (by injection; positive controls) and glycerol with water only (by embrocation; negative controls), respectively. Each rabbit was treated twice with separate treatments on days 0 and 7. Rabbits were observed daily and detailed examinations were performed on days 0, 7 and 14, to inspect the presence or absence of mites and scabs/crusts. Seven days after the initial treatment, the mean clinical scores (presence of scabs/crusts) decreased from 3.48, 3.37, 3.43 and 3.45 to 0.37, 0.42, 0.78 and 0.38 in the ears of animals in groups A, B , C and D, respectively, which were similar to the observations recorded in the positive control rabbits. However, the clinical score for negative control rabbits did not increase significantly (P > 0.05) during the experiment, and this changed from 3.32 to 3.37 in the ears, and there were no significant differences in clinical efficacy between left and right ears. After two treatments (0 and 7 d), the rabbits in groups A, B, C and D had recovered completely 14 days after the last treatment and no recurrences of infection were observed. CONCLUSIONS: These results indicate that euptox A was potent compounds for the effective control of animal P. cuniculi in vivo.
format Online
Article
Text
id pubmed-4308076
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-43080762015-01-28 Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits Hu, Yang Liao, Fei Hu, Yanchun Luo, Biao He, Yajun Mo, Quan Zuo, Zhicai Ren, Zhihua Deng, Junliang Wei, Yahui BMC Vet Res Research Article BACKGROUND: Animal acariasis is one of the important veterinary skin diseases. Chemical drugs have been widely used to treat and control this kind of disease. But many chemicals control could increase resistance in target species, toxicity and environmental hazards. We found that the 9-oxo-10, 11-dehydroageraphorone (euptox A) extracted from E. adenophorum has strong toxicity against P. cuniculi in vitro, but the in vivo acaricidal actions of euptox A have yet to be investigated. RESULTS: A 14-day experiment was performed using rabbits that were naturally infested with P. cuniculi on a farm. Rabbits were randomly divided into five groups; animals in groups A, B and C were treated in each ear topically with 4.0 ml of 2.0 and 1.0 g/L (w/v) euptox A, respectively. Animals in groups D and E were treated with ivermectin (by injection; positive controls) and glycerol with water only (by embrocation; negative controls), respectively. Each rabbit was treated twice with separate treatments on days 0 and 7. Rabbits were observed daily and detailed examinations were performed on days 0, 7 and 14, to inspect the presence or absence of mites and scabs/crusts. Seven days after the initial treatment, the mean clinical scores (presence of scabs/crusts) decreased from 3.48, 3.37, 3.43 and 3.45 to 0.37, 0.42, 0.78 and 0.38 in the ears of animals in groups A, B , C and D, respectively, which were similar to the observations recorded in the positive control rabbits. However, the clinical score for negative control rabbits did not increase significantly (P > 0.05) during the experiment, and this changed from 3.32 to 3.37 in the ears, and there were no significant differences in clinical efficacy between left and right ears. After two treatments (0 and 7 d), the rabbits in groups A, B, C and D had recovered completely 14 days after the last treatment and no recurrences of infection were observed. CONCLUSIONS: These results indicate that euptox A was potent compounds for the effective control of animal P. cuniculi in vivo. BioMed Central 2014-12-20 /pmc/articles/PMC4308076/ /pubmed/25527276 http://dx.doi.org/10.1186/s12917-014-0306-4 Text en © Hu et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Hu, Yang
Liao, Fei
Hu, Yanchun
Luo, Biao
He, Yajun
Mo, Quan
Zuo, Zhicai
Ren, Zhihua
Deng, Junliang
Wei, Yahui
Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits
title Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits
title_full Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits
title_fullStr Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits
title_full_unstemmed Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits
title_short Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits
title_sort clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from eupatorium adenophorum against psoroptes cuniculi in rabbits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308076/
https://www.ncbi.nlm.nih.gov/pubmed/25527276
http://dx.doi.org/10.1186/s12917-014-0306-4
work_keys_str_mv AT huyang clinicalefficacyof9oxo1011dehydroageraphoroneextractedfromeupatoriumadenophorumagainstpsoroptescuniculiinrabbits
AT liaofei clinicalefficacyof9oxo1011dehydroageraphoroneextractedfromeupatoriumadenophorumagainstpsoroptescuniculiinrabbits
AT huyanchun clinicalefficacyof9oxo1011dehydroageraphoroneextractedfromeupatoriumadenophorumagainstpsoroptescuniculiinrabbits
AT luobiao clinicalefficacyof9oxo1011dehydroageraphoroneextractedfromeupatoriumadenophorumagainstpsoroptescuniculiinrabbits
AT heyajun clinicalefficacyof9oxo1011dehydroageraphoroneextractedfromeupatoriumadenophorumagainstpsoroptescuniculiinrabbits
AT moquan clinicalefficacyof9oxo1011dehydroageraphoroneextractedfromeupatoriumadenophorumagainstpsoroptescuniculiinrabbits
AT zuozhicai clinicalefficacyof9oxo1011dehydroageraphoroneextractedfromeupatoriumadenophorumagainstpsoroptescuniculiinrabbits
AT renzhihua clinicalefficacyof9oxo1011dehydroageraphoroneextractedfromeupatoriumadenophorumagainstpsoroptescuniculiinrabbits
AT dengjunliang clinicalefficacyof9oxo1011dehydroageraphoroneextractedfromeupatoriumadenophorumagainstpsoroptescuniculiinrabbits
AT weiyahui clinicalefficacyof9oxo1011dehydroageraphoroneextractedfromeupatoriumadenophorumagainstpsoroptescuniculiinrabbits