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Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)

Insect chemosensory proteins (CSPs) are a family of small soluble proteins. To date, their physiological functions in insect olfaction remain largely controversial in comparison to odorant binding proteins (OBPs). In present study, we reported the antenna specific expression of three CSPs (AlinCSP4-...

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Autores principales: Sun, Liang, Zhou, Jing-Jiang, Gu, Shao-Hua, Xiao, Hai-Jun, Guo, Yu-Yuan, Liu, Ze-Wen, Zhang, Yong-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308698/
https://www.ncbi.nlm.nih.gov/pubmed/25627422
http://dx.doi.org/10.1038/srep08073
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author Sun, Liang
Zhou, Jing-Jiang
Gu, Shao-Hua
Xiao, Hai-Jun
Guo, Yu-Yuan
Liu, Ze-Wen
Zhang, Yong-Jun
author_facet Sun, Liang
Zhou, Jing-Jiang
Gu, Shao-Hua
Xiao, Hai-Jun
Guo, Yu-Yuan
Liu, Ze-Wen
Zhang, Yong-Jun
author_sort Sun, Liang
collection PubMed
description Insect chemosensory proteins (CSPs) are a family of small soluble proteins. To date, their physiological functions in insect olfaction remain largely controversial in comparison to odorant binding proteins (OBPs). In present study, we reported the antenna specific expression of three CSPs (AlinCSP4-6) from Adelphocoris lineolatus, their distinct chemosensillum distribution as well as ligand binding capability thus providing the evidence for the possible roles that they could play in semiochemical detection of the plant bug A. lineolatus. The results of qRT-PCR and western blot assay clearly showed that all of these three CSPs are highly expressed in the adult antennae, the olfactory organ of insects. Further cellular investigation of their immunolocalization revealed their dynamic protein expression profiles among different types of antennal sensilla. In a fluorescence competitive binding assay, the selective ligand binding was observed for AlinCSP4-6. In ad`dition, a cooperative interaction was observed between two co-expressed CSPs resulting in an increase of the binding affinities by a mixture of AlinCSP5 and AlinCSP6 to terpenoids which do not bind to individual CSPs. These findings in combination with our previous data for AlinCSP1-3 indicate a possible functional differentiation of CSPs in the A. lineolatus olfactory system.
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spelling pubmed-43086982015-02-06 Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze) Sun, Liang Zhou, Jing-Jiang Gu, Shao-Hua Xiao, Hai-Jun Guo, Yu-Yuan Liu, Ze-Wen Zhang, Yong-Jun Sci Rep Article Insect chemosensory proteins (CSPs) are a family of small soluble proteins. To date, their physiological functions in insect olfaction remain largely controversial in comparison to odorant binding proteins (OBPs). In present study, we reported the antenna specific expression of three CSPs (AlinCSP4-6) from Adelphocoris lineolatus, their distinct chemosensillum distribution as well as ligand binding capability thus providing the evidence for the possible roles that they could play in semiochemical detection of the plant bug A. lineolatus. The results of qRT-PCR and western blot assay clearly showed that all of these three CSPs are highly expressed in the adult antennae, the olfactory organ of insects. Further cellular investigation of their immunolocalization revealed their dynamic protein expression profiles among different types of antennal sensilla. In a fluorescence competitive binding assay, the selective ligand binding was observed for AlinCSP4-6. In ad`dition, a cooperative interaction was observed between two co-expressed CSPs resulting in an increase of the binding affinities by a mixture of AlinCSP5 and AlinCSP6 to terpenoids which do not bind to individual CSPs. These findings in combination with our previous data for AlinCSP1-3 indicate a possible functional differentiation of CSPs in the A. lineolatus olfactory system. Nature Publishing Group 2015-01-28 /pmc/articles/PMC4308698/ /pubmed/25627422 http://dx.doi.org/10.1038/srep08073 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Sun, Liang
Zhou, Jing-Jiang
Gu, Shao-Hua
Xiao, Hai-Jun
Guo, Yu-Yuan
Liu, Ze-Wen
Zhang, Yong-Jun
Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)
title Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)
title_full Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)
title_fullStr Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)
title_full_unstemmed Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)
title_short Chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug Adelphocoris lineolatus (Goeze)
title_sort chemosensillum immunolocalization and ligand specificity of chemosensory proteins in the alfalfa plant bug adelphocoris lineolatus (goeze)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308698/
https://www.ncbi.nlm.nih.gov/pubmed/25627422
http://dx.doi.org/10.1038/srep08073
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