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AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis

Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate tran...

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Autores principales: Miyaji, Takaaki, Kuromori, Takashi, Takeuchi, Yu, Yamaji, Naoki, Yokosho, Kengo, Shimazawa, Atsushi, Sugimoto, Eriko, Omote, Hiroshi, Ma, Jian Feng, Shinozaki, Kazuo, Moriyama, Yoshinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308718/
https://www.ncbi.nlm.nih.gov/pubmed/25557369
http://dx.doi.org/10.1038/ncomms6928
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author Miyaji, Takaaki
Kuromori, Takashi
Takeuchi, Yu
Yamaji, Naoki
Yokosho, Kengo
Shimazawa, Atsushi
Sugimoto, Eriko
Omote, Hiroshi
Ma, Jian Feng
Shinozaki, Kazuo
Moriyama, Yoshinori
author_facet Miyaji, Takaaki
Kuromori, Takashi
Takeuchi, Yu
Yamaji, Naoki
Yokosho, Kengo
Shimazawa, Atsushi
Sugimoto, Eriko
Omote, Hiroshi
Ma, Jian Feng
Shinozaki, Kazuo
Moriyama, Yoshinori
author_sort Miyaji, Takaaki
collection PubMed
description Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl(−)-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress.
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spelling pubmed-43087182015-02-09 AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis Miyaji, Takaaki Kuromori, Takashi Takeuchi, Yu Yamaji, Naoki Yokosho, Kengo Shimazawa, Atsushi Sugimoto, Eriko Omote, Hiroshi Ma, Jian Feng Shinozaki, Kazuo Moriyama, Yoshinori Nat Commun Article Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl(−)-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress. Nature Pub. Group 2015-01-05 /pmc/articles/PMC4308718/ /pubmed/25557369 http://dx.doi.org/10.1038/ncomms6928 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Miyaji, Takaaki
Kuromori, Takashi
Takeuchi, Yu
Yamaji, Naoki
Yokosho, Kengo
Shimazawa, Atsushi
Sugimoto, Eriko
Omote, Hiroshi
Ma, Jian Feng
Shinozaki, Kazuo
Moriyama, Yoshinori
AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis
title AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis
title_full AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis
title_fullStr AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis
title_full_unstemmed AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis
title_short AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis
title_sort atpht4;4 is a chloroplast-localized ascorbate transporter in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308718/
https://www.ncbi.nlm.nih.gov/pubmed/25557369
http://dx.doi.org/10.1038/ncomms6928
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