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AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis
Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate tran...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308718/ https://www.ncbi.nlm.nih.gov/pubmed/25557369 http://dx.doi.org/10.1038/ncomms6928 |
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author | Miyaji, Takaaki Kuromori, Takashi Takeuchi, Yu Yamaji, Naoki Yokosho, Kengo Shimazawa, Atsushi Sugimoto, Eriko Omote, Hiroshi Ma, Jian Feng Shinozaki, Kazuo Moriyama, Yoshinori |
author_facet | Miyaji, Takaaki Kuromori, Takashi Takeuchi, Yu Yamaji, Naoki Yokosho, Kengo Shimazawa, Atsushi Sugimoto, Eriko Omote, Hiroshi Ma, Jian Feng Shinozaki, Kazuo Moriyama, Yoshinori |
author_sort | Miyaji, Takaaki |
collection | PubMed |
description | Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl(−)-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress. |
format | Online Article Text |
id | pubmed-4308718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43087182015-02-09 AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis Miyaji, Takaaki Kuromori, Takashi Takeuchi, Yu Yamaji, Naoki Yokosho, Kengo Shimazawa, Atsushi Sugimoto, Eriko Omote, Hiroshi Ma, Jian Feng Shinozaki, Kazuo Moriyama, Yoshinori Nat Commun Article Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl(−)-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress. Nature Pub. Group 2015-01-05 /pmc/articles/PMC4308718/ /pubmed/25557369 http://dx.doi.org/10.1038/ncomms6928 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Miyaji, Takaaki Kuromori, Takashi Takeuchi, Yu Yamaji, Naoki Yokosho, Kengo Shimazawa, Atsushi Sugimoto, Eriko Omote, Hiroshi Ma, Jian Feng Shinozaki, Kazuo Moriyama, Yoshinori AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis |
title | AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis |
title_full | AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis |
title_fullStr | AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis |
title_full_unstemmed | AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis |
title_short | AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis |
title_sort | atpht4;4 is a chloroplast-localized ascorbate transporter in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308718/ https://www.ncbi.nlm.nih.gov/pubmed/25557369 http://dx.doi.org/10.1038/ncomms6928 |
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