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Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker),...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308779/ https://www.ncbi.nlm.nih.gov/pubmed/25657683 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.19.006 |
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author | Zhu, Guowei Sun, Chongran Liu, Weiguo |
author_facet | Zhu, Guowei Sun, Chongran Liu, Weiguo |
author_sort | Zhu, Guowei |
collection | PubMed |
description | In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker), and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for O(4) (oligodendrocytic marker). At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O(4)-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes. |
format | Online Article Text |
id | pubmed-4308779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-43087792015-02-05 Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes Zhu, Guowei Sun, Chongran Liu, Weiguo Neural Regen Res Research and Report: Stem Cells and Neural Regeneration In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker), and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for O(4) (oligodendrocytic marker). At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O(4)-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes. Medknow Publications & Media Pvt Ltd 2012-07-05 /pmc/articles/PMC4308779/ /pubmed/25657683 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.19.006 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research and Report: Stem Cells and Neural Regeneration Zhu, Guowei Sun, Chongran Liu, Weiguo Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes |
title | Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes |
title_full | Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes |
title_fullStr | Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes |
title_full_unstemmed | Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes |
title_short | Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes |
title_sort | effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes |
topic | Research and Report: Stem Cells and Neural Regeneration |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308779/ https://www.ncbi.nlm.nih.gov/pubmed/25657683 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.19.006 |
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