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Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes

In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker),...

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Detalles Bibliográficos
Autores principales: Zhu, Guowei, Sun, Chongran, Liu, Weiguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308779/
https://www.ncbi.nlm.nih.gov/pubmed/25657683
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.19.006
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author Zhu, Guowei
Sun, Chongran
Liu, Weiguo
author_facet Zhu, Guowei
Sun, Chongran
Liu, Weiguo
author_sort Zhu, Guowei
collection PubMed
description In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker), and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for O(4) (oligodendrocytic marker). At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O(4)-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.
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spelling pubmed-43087792015-02-05 Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes Zhu, Guowei Sun, Chongran Liu, Weiguo Neural Regen Res Research and Report: Stem Cells and Neural Regeneration In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker), and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for O(4) (oligodendrocytic marker). At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O(4)-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes. Medknow Publications & Media Pvt Ltd 2012-07-05 /pmc/articles/PMC4308779/ /pubmed/25657683 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.19.006 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report: Stem Cells and Neural Regeneration
Zhu, Guowei
Sun, Chongran
Liu, Weiguo
Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
title Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
title_full Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
title_fullStr Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
title_full_unstemmed Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
title_short Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
title_sort effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes
topic Research and Report: Stem Cells and Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308779/
https://www.ncbi.nlm.nih.gov/pubmed/25657683
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.19.006
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