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The 9L(LUC)/Wistar rat glioma model is not suitable for immunotherapy☆

The availability of a well-characterized animal brain tumor model will play an important role in identifying treatments for human brain tumors. Wistar rats bearing 9L glioma cells can develop solid, well-circumcised tumors, and may be a useful animal model for the evaluation of various therapeutic a...

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Autores principales: Yang, Liping, Zhao, Jingxiang, Zhou, Guihong, Wang, Yunfang, Li, Lusi, Yuan, Hongfeng, Nan, Xue, Guan, Lidong, Pei, Xuetao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308791/
https://www.ncbi.nlm.nih.gov/pubmed/25657674
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.18.007
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author Yang, Liping
Zhao, Jingxiang
Zhou, Guihong
Wang, Yunfang
Li, Lusi
Yuan, Hongfeng
Nan, Xue
Guan, Lidong
Pei, Xuetao
author_facet Yang, Liping
Zhao, Jingxiang
Zhou, Guihong
Wang, Yunfang
Li, Lusi
Yuan, Hongfeng
Nan, Xue
Guan, Lidong
Pei, Xuetao
author_sort Yang, Liping
collection PubMed
description The availability of a well-characterized animal brain tumor model will play an important role in identifying treatments for human brain tumors. Wistar rats bearing 9L glioma cells can develop solid, well-circumcised tumors, and may be a useful animal model for the evaluation of various therapeutic approaches for gliosarcomas. In this study, the 9L/Wistar rat glioma model was produced by intracerebral implantation of 9L(LUC) glioma cells syngenic to Fischer 344 (F344) rats. Bioluminescence imaging showed that tumors progressively grew from day 7 to day 21 in 9L(LUC)/F344 rats, and tumor regression was found in some 9L(LUC)/Wistar rats. Hematoxylin-eosin staining verified that intracranial tumors were gliomas. Immunohistochemistry results demonstrated that no CD(4)- and CD(8)-positive cells were found in the syngeneic 9L(LUC)/F344 model. However, many infiltrating CD(4)- and CD(8)-positive cells were observed within the tumors of the 9L(LUC)/Wistar model. Our data suggests that compared with 9L/F344 rats, 9L glioma Wistar rats may not be suitable for evaluating brain glioma immunotherapies, even though the model induced an immune response and exhibited tumor regression.
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spelling pubmed-43087912015-02-05 The 9L(LUC)/Wistar rat glioma model is not suitable for immunotherapy☆ Yang, Liping Zhao, Jingxiang Zhou, Guihong Wang, Yunfang Li, Lusi Yuan, Hongfeng Nan, Xue Guan, Lidong Pei, Xuetao Neural Regen Res Techniques and Method: Emerging Technology in Neural Regeneration The availability of a well-characterized animal brain tumor model will play an important role in identifying treatments for human brain tumors. Wistar rats bearing 9L glioma cells can develop solid, well-circumcised tumors, and may be a useful animal model for the evaluation of various therapeutic approaches for gliosarcomas. In this study, the 9L/Wistar rat glioma model was produced by intracerebral implantation of 9L(LUC) glioma cells syngenic to Fischer 344 (F344) rats. Bioluminescence imaging showed that tumors progressively grew from day 7 to day 21 in 9L(LUC)/F344 rats, and tumor regression was found in some 9L(LUC)/Wistar rats. Hematoxylin-eosin staining verified that intracranial tumors were gliomas. Immunohistochemistry results demonstrated that no CD(4)- and CD(8)-positive cells were found in the syngeneic 9L(LUC)/F344 model. However, many infiltrating CD(4)- and CD(8)-positive cells were observed within the tumors of the 9L(LUC)/Wistar model. Our data suggests that compared with 9L/F344 rats, 9L glioma Wistar rats may not be suitable for evaluating brain glioma immunotherapies, even though the model induced an immune response and exhibited tumor regression. Medknow Publications & Media Pvt Ltd 2012-06-25 /pmc/articles/PMC4308791/ /pubmed/25657674 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.18.007 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Techniques and Method: Emerging Technology in Neural Regeneration
Yang, Liping
Zhao, Jingxiang
Zhou, Guihong
Wang, Yunfang
Li, Lusi
Yuan, Hongfeng
Nan, Xue
Guan, Lidong
Pei, Xuetao
The 9L(LUC)/Wistar rat glioma model is not suitable for immunotherapy☆
title The 9L(LUC)/Wistar rat glioma model is not suitable for immunotherapy☆
title_full The 9L(LUC)/Wistar rat glioma model is not suitable for immunotherapy☆
title_fullStr The 9L(LUC)/Wistar rat glioma model is not suitable for immunotherapy☆
title_full_unstemmed The 9L(LUC)/Wistar rat glioma model is not suitable for immunotherapy☆
title_short The 9L(LUC)/Wistar rat glioma model is not suitable for immunotherapy☆
title_sort 9l(luc)/wistar rat glioma model is not suitable for immunotherapy☆
topic Techniques and Method: Emerging Technology in Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308791/
https://www.ncbi.nlm.nih.gov/pubmed/25657674
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.18.007
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