Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells☆

A human SH-SY5Y neuroblastoma cell line with a low level of Bax inhibitor-1 expression was established by lentivirus-mediated RNA interference and fluorescence-activated cell sorting. In control SH-SY5Y cells, tunicamycin treatment induced endoplasmic reticulum stress-mediated apoptosis; however, af...

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Detalles Bibliográficos
Autores principales: Wu, Dan, Wang, Peirong, Wang, Shiyao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Research and Report: Peripheral Nerve Injury and Neural Regeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308804/
https://www.ncbi.nlm.nih.gov/pubmed/25657664
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.17.008
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author Wu, Dan
Wang, Peirong
Wang, Shiyao
author_facet Wu, Dan
Wang, Peirong
Wang, Shiyao
author_sort Wu, Dan
collection PubMed
description A human SH-SY5Y neuroblastoma cell line with a low level of Bax inhibitor-1 expression was established by lentivirus-mediated RNA interference and fluorescence-activated cell sorting. In control SH-SY5Y cells, tunicamycin treatment induced endoplasmic reticulum stress-mediated apoptosis; however, after Bax inhibitor-1 gene knockdown, cell survival rates were significantly decreased and the degree of apoptosis was significantly increased following tunicamycin treatment. In addition, chromatin condensation and apparent apoptotic phenomena, such as marginalization and cytoplasmic vesicles, were observed. Our findings indicate that Bax inhibitor-1 can delay apoptosis induced by endoplasmic reticulum stress.
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spelling pubmed-43088042015-02-05 Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells☆ Wu, Dan Wang, Peirong Wang, Shiyao Neural Regen Res Research and Report: Peripheral Nerve Injury and Neural Regeneration A human SH-SY5Y neuroblastoma cell line with a low level of Bax inhibitor-1 expression was established by lentivirus-mediated RNA interference and fluorescence-activated cell sorting. In control SH-SY5Y cells, tunicamycin treatment induced endoplasmic reticulum stress-mediated apoptosis; however, after Bax inhibitor-1 gene knockdown, cell survival rates were significantly decreased and the degree of apoptosis was significantly increased following tunicamycin treatment. In addition, chromatin condensation and apparent apoptotic phenomena, such as marginalization and cytoplasmic vesicles, were observed. Our findings indicate that Bax inhibitor-1 can delay apoptosis induced by endoplasmic reticulum stress. Medknow Publications & Media Pvt Ltd 2012-06-15 /pmc/articles/PMC4308804/ /pubmed/25657664 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.17.008 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report: Peripheral Nerve Injury and Neural Regeneration
Wu, Dan
Wang, Peirong
Wang, Shiyao
Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells☆
title Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells☆
title_full Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells☆
title_fullStr Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells☆
title_full_unstemmed Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells☆
title_short Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells☆
title_sort low levels of bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma sy5y cells☆
topic Research and Report: Peripheral Nerve Injury and Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308804/
https://www.ncbi.nlm.nih.gov/pubmed/25657664
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.17.008
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AT wangshiyao lowlevelsofbaxinhibitor1geneexpressionincreasetunicamycininducedapoptosisinhumanneuroblastomasy5ycells

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