Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury★

In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury....

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Autores principales: Li, Junliang, Xu, Xinke, Zhang, Shanyi, Zheng, Meiguang, Wu, Zhonghua, Weng, Yinlun, Ouyang, Leping, Yu, Jian, Li, Fangcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Research and Report: Peripheral Nerve Injury and Neural Regeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308805/
https://www.ncbi.nlm.nih.gov/pubmed/25657665
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.17.009
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author Li, Junliang
Xu, Xinke
Zhang, Shanyi
Zheng, Meiguang
Wu, Zhonghua
Weng, Yinlun
Ouyang, Leping
Yu, Jian
Li, Fangcheng
author_facet Li, Junliang
Xu, Xinke
Zhang, Shanyi
Zheng, Meiguang
Wu, Zhonghua
Weng, Yinlun
Ouyang, Leping
Yu, Jian
Li, Fangcheng
author_sort Li, Junliang
collection PubMed
description In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury. The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3. These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.
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spelling pubmed-43088052015-02-05 Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury★ Li, Junliang Xu, Xinke Zhang, Shanyi Zheng, Meiguang Wu, Zhonghua Weng, Yinlun Ouyang, Leping Yu, Jian Li, Fangcheng Neural Regen Res Research and Report: Peripheral Nerve Injury and Neural Regeneration In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury. The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3. These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis. Medknow Publications & Media Pvt Ltd 2012-06-15 /pmc/articles/PMC4308805/ /pubmed/25657665 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.17.009 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report: Peripheral Nerve Injury and Neural Regeneration
Li, Junliang
Xu, Xinke
Zhang, Shanyi
Zheng, Meiguang
Wu, Zhonghua
Weng, Yinlun
Ouyang, Leping
Yu, Jian
Li, Fangcheng
Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury★
title Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury★
title_full Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury★
title_fullStr Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury★
title_full_unstemmed Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury★
title_short Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury★
title_sort adenovirus-mediated transfection with glucose transporter 3 suppresses pc12 cell apoptosis following ischemic injury★
topic Research and Report: Peripheral Nerve Injury and Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308805/
https://www.ncbi.nlm.nih.gov/pubmed/25657665
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.17.009
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