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Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity
BACKGROUND: Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308894/ https://www.ncbi.nlm.nih.gov/pubmed/25632299 http://dx.doi.org/10.1186/s13068-014-0184-9 |
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author | Muto, Masaki Tanaka, Masayoshi Liang, Yue Yoshino, Tomoko Matsumoto, Mitsufumi Tanaka, Tsuyoshi |
author_facet | Muto, Masaki Tanaka, Masayoshi Liang, Yue Yoshino, Tomoko Matsumoto, Mitsufumi Tanaka, Tsuyoshi |
author_sort | Muto, Masaki |
collection | PubMed |
description | BACKGROUND: Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity and biomass productivity by overcoming the growth retardation caused by decreased light penetration in high-density culture. RESULTS: Overexpression of the endogenous glycerol kinase (GK) gene in an oleaginous marine diatom, Fistulifera solaris JPCC DA0580, accelerates glycerol metabolism and improves lipid and biomass productivities. Two candidates were selected from a collection of 90 G418-resistant clones, based on growth and confirmation of genome integration. GK gene expression was higher in the selected clones (GK1_7 and GK2_16) than in the wild-type culture. The GK2_16 clone achieved a 12% increase in lipid productivity. CONCLUSION: We have demonstrated the potential of metabolic engineering in oleaginous microalgae to improve lipid productivity. Metabolic engineering techniques can be used to optimize BDF production. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-014-0184-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4308894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43088942015-01-29 Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity Muto, Masaki Tanaka, Masayoshi Liang, Yue Yoshino, Tomoko Matsumoto, Mitsufumi Tanaka, Tsuyoshi Biotechnol Biofuels Research Article BACKGROUND: Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity and biomass productivity by overcoming the growth retardation caused by decreased light penetration in high-density culture. RESULTS: Overexpression of the endogenous glycerol kinase (GK) gene in an oleaginous marine diatom, Fistulifera solaris JPCC DA0580, accelerates glycerol metabolism and improves lipid and biomass productivities. Two candidates were selected from a collection of 90 G418-resistant clones, based on growth and confirmation of genome integration. GK gene expression was higher in the selected clones (GK1_7 and GK2_16) than in the wild-type culture. The GK2_16 clone achieved a 12% increase in lipid productivity. CONCLUSION: We have demonstrated the potential of metabolic engineering in oleaginous microalgae to improve lipid productivity. Metabolic engineering techniques can be used to optimize BDF production. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-014-0184-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-01-22 /pmc/articles/PMC4308894/ /pubmed/25632299 http://dx.doi.org/10.1186/s13068-014-0184-9 Text en © Muto et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Muto, Masaki Tanaka, Masayoshi Liang, Yue Yoshino, Tomoko Matsumoto, Mitsufumi Tanaka, Tsuyoshi Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity |
title | Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity |
title_full | Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity |
title_fullStr | Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity |
title_full_unstemmed | Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity |
title_short | Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity |
title_sort | enhancement of glycerol metabolism in the oleaginous marine diatom fistulifera solaris jpcc da0580 to improve triacylglycerol productivity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308894/ https://www.ncbi.nlm.nih.gov/pubmed/25632299 http://dx.doi.org/10.1186/s13068-014-0184-9 |
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