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Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity

BACKGROUND: Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity...

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Autores principales: Muto, Masaki, Tanaka, Masayoshi, Liang, Yue, Yoshino, Tomoko, Matsumoto, Mitsufumi, Tanaka, Tsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308894/
https://www.ncbi.nlm.nih.gov/pubmed/25632299
http://dx.doi.org/10.1186/s13068-014-0184-9
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author Muto, Masaki
Tanaka, Masayoshi
Liang, Yue
Yoshino, Tomoko
Matsumoto, Mitsufumi
Tanaka, Tsuyoshi
author_facet Muto, Masaki
Tanaka, Masayoshi
Liang, Yue
Yoshino, Tomoko
Matsumoto, Mitsufumi
Tanaka, Tsuyoshi
author_sort Muto, Masaki
collection PubMed
description BACKGROUND: Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity and biomass productivity by overcoming the growth retardation caused by decreased light penetration in high-density culture. RESULTS: Overexpression of the endogenous glycerol kinase (GK) gene in an oleaginous marine diatom, Fistulifera solaris JPCC DA0580, accelerates glycerol metabolism and improves lipid and biomass productivities. Two candidates were selected from a collection of 90 G418-resistant clones, based on growth and confirmation of genome integration. GK gene expression was higher in the selected clones (GK1_7 and GK2_16) than in the wild-type culture. The GK2_16 clone achieved a 12% increase in lipid productivity. CONCLUSION: We have demonstrated the potential of metabolic engineering in oleaginous microalgae to improve lipid productivity. Metabolic engineering techniques can be used to optimize BDF production. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-014-0184-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-43088942015-01-29 Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity Muto, Masaki Tanaka, Masayoshi Liang, Yue Yoshino, Tomoko Matsumoto, Mitsufumi Tanaka, Tsuyoshi Biotechnol Biofuels Research Article BACKGROUND: Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity and biomass productivity by overcoming the growth retardation caused by decreased light penetration in high-density culture. RESULTS: Overexpression of the endogenous glycerol kinase (GK) gene in an oleaginous marine diatom, Fistulifera solaris JPCC DA0580, accelerates glycerol metabolism and improves lipid and biomass productivities. Two candidates were selected from a collection of 90 G418-resistant clones, based on growth and confirmation of genome integration. GK gene expression was higher in the selected clones (GK1_7 and GK2_16) than in the wild-type culture. The GK2_16 clone achieved a 12% increase in lipid productivity. CONCLUSION: We have demonstrated the potential of metabolic engineering in oleaginous microalgae to improve lipid productivity. Metabolic engineering techniques can be used to optimize BDF production. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-014-0184-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-01-22 /pmc/articles/PMC4308894/ /pubmed/25632299 http://dx.doi.org/10.1186/s13068-014-0184-9 Text en © Muto et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Muto, Masaki
Tanaka, Masayoshi
Liang, Yue
Yoshino, Tomoko
Matsumoto, Mitsufumi
Tanaka, Tsuyoshi
Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity
title Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity
title_full Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity
title_fullStr Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity
title_full_unstemmed Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity
title_short Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity
title_sort enhancement of glycerol metabolism in the oleaginous marine diatom fistulifera solaris jpcc da0580 to improve triacylglycerol productivity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308894/
https://www.ncbi.nlm.nih.gov/pubmed/25632299
http://dx.doi.org/10.1186/s13068-014-0184-9
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