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Genes Required for Survival in Microgravity Revealed by Genome-Wide Yeast Deletion Collections Cultured during Spaceflight

Spaceflight is a unique environment with profound effects on biological systems including tissue redistribution and musculoskeletal stresses. However, the more subtle biological effects of spaceflight on cells and organisms are difficult to measure in a systematic, unbiased manner. Here we test the...

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Autores principales: Nislow, Corey, Lee, Anna Y., Allen, Patricia L., Giaever, Guri, Smith, Andrew, Gebbia, Marinella, Stodieck, Louis S., Hammond, Jeffrey S., Birdsall, Holly H., Hammond, Timothy G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309212/
https://www.ncbi.nlm.nih.gov/pubmed/25667933
http://dx.doi.org/10.1155/2015/976458
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author Nislow, Corey
Lee, Anna Y.
Allen, Patricia L.
Giaever, Guri
Smith, Andrew
Gebbia, Marinella
Stodieck, Louis S.
Hammond, Jeffrey S.
Birdsall, Holly H.
Hammond, Timothy G.
author_facet Nislow, Corey
Lee, Anna Y.
Allen, Patricia L.
Giaever, Guri
Smith, Andrew
Gebbia, Marinella
Stodieck, Louis S.
Hammond, Jeffrey S.
Birdsall, Holly H.
Hammond, Timothy G.
author_sort Nislow, Corey
collection PubMed
description Spaceflight is a unique environment with profound effects on biological systems including tissue redistribution and musculoskeletal stresses. However, the more subtle biological effects of spaceflight on cells and organisms are difficult to measure in a systematic, unbiased manner. Here we test the utility of the molecularly barcoded yeast deletion collection to provide a quantitative assessment of the effects of microgravity on a model organism. We developed robust hardware to screen, in parallel, the complete collection of ~4800 homozygous and ~5900 heterozygous (including ~1100 single-copy deletions of essential genes) yeast deletion strains, each carrying unique DNA that acts as strain identifiers. We compared strain fitness for the homozygous and heterozygous yeast deletion collections grown in spaceflight and ground, as well as plus and minus hyperosmolar sodium chloride, providing a second additive stressor. The genome-wide sensitivity profiles obtained from these treatments were then queried for their similarity to a compendium of drugs whose effects on the yeast collection have been previously reported. We found that the effects of spaceflight have high concordance with the effects of DNA-damaging agents and changes in redox state, suggesting mechanisms by which spaceflight may negatively affect cell fitness.
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spelling pubmed-43092122015-02-09 Genes Required for Survival in Microgravity Revealed by Genome-Wide Yeast Deletion Collections Cultured during Spaceflight Nislow, Corey Lee, Anna Y. Allen, Patricia L. Giaever, Guri Smith, Andrew Gebbia, Marinella Stodieck, Louis S. Hammond, Jeffrey S. Birdsall, Holly H. Hammond, Timothy G. Biomed Res Int Research Article Spaceflight is a unique environment with profound effects on biological systems including tissue redistribution and musculoskeletal stresses. However, the more subtle biological effects of spaceflight on cells and organisms are difficult to measure in a systematic, unbiased manner. Here we test the utility of the molecularly barcoded yeast deletion collection to provide a quantitative assessment of the effects of microgravity on a model organism. We developed robust hardware to screen, in parallel, the complete collection of ~4800 homozygous and ~5900 heterozygous (including ~1100 single-copy deletions of essential genes) yeast deletion strains, each carrying unique DNA that acts as strain identifiers. We compared strain fitness for the homozygous and heterozygous yeast deletion collections grown in spaceflight and ground, as well as plus and minus hyperosmolar sodium chloride, providing a second additive stressor. The genome-wide sensitivity profiles obtained from these treatments were then queried for their similarity to a compendium of drugs whose effects on the yeast collection have been previously reported. We found that the effects of spaceflight have high concordance with the effects of DNA-damaging agents and changes in redox state, suggesting mechanisms by which spaceflight may negatively affect cell fitness. Hindawi Publishing Corporation 2015 2015-01-13 /pmc/articles/PMC4309212/ /pubmed/25667933 http://dx.doi.org/10.1155/2015/976458 Text en Copyright © 2015 Corey Nislow et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nislow, Corey
Lee, Anna Y.
Allen, Patricia L.
Giaever, Guri
Smith, Andrew
Gebbia, Marinella
Stodieck, Louis S.
Hammond, Jeffrey S.
Birdsall, Holly H.
Hammond, Timothy G.
Genes Required for Survival in Microgravity Revealed by Genome-Wide Yeast Deletion Collections Cultured during Spaceflight
title Genes Required for Survival in Microgravity Revealed by Genome-Wide Yeast Deletion Collections Cultured during Spaceflight
title_full Genes Required for Survival in Microgravity Revealed by Genome-Wide Yeast Deletion Collections Cultured during Spaceflight
title_fullStr Genes Required for Survival in Microgravity Revealed by Genome-Wide Yeast Deletion Collections Cultured during Spaceflight
title_full_unstemmed Genes Required for Survival in Microgravity Revealed by Genome-Wide Yeast Deletion Collections Cultured during Spaceflight
title_short Genes Required for Survival in Microgravity Revealed by Genome-Wide Yeast Deletion Collections Cultured during Spaceflight
title_sort genes required for survival in microgravity revealed by genome-wide yeast deletion collections cultured during spaceflight
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309212/
https://www.ncbi.nlm.nih.gov/pubmed/25667933
http://dx.doi.org/10.1155/2015/976458
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