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Beneficial Effect of Sugar Osmolytes on the Refolding of Guanidine Hydrochloride-Denatured Trehalose-6-phosphate Hydrolase from Bacillus licheniformis

The influence of three sugar osmolytes on the refolding of guanidine hydrochloride- (GdnHCl-) denatured trehalose-6-phosphate hydrolase of Bacillus licheniformis (BlTreA) was studied by circular dichroism (CD) spectra, fluorescence emission spectra, and the recovery of enzymatic activity. These expe...

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Detalles Bibliográficos
Autores principales: Chen, Jiau-Hua, Chi, Meng-Chun, Lin, Min-Guan, Lin, Long-Liu, Wang, Tzu-Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309298/
https://www.ncbi.nlm.nih.gov/pubmed/25667926
http://dx.doi.org/10.1155/2015/806847

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