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Beneficial Effect of Sugar Osmolytes on the Refolding of Guanidine Hydrochloride-Denatured Trehalose-6-phosphate Hydrolase from Bacillus licheniformis
The influence of three sugar osmolytes on the refolding of guanidine hydrochloride- (GdnHCl-) denatured trehalose-6-phosphate hydrolase of Bacillus licheniformis (BlTreA) was studied by circular dichroism (CD) spectra, fluorescence emission spectra, and the recovery of enzymatic activity. These expe...
Autores principales: | Chen, Jiau-Hua, Chi, Meng-Chun, Lin, Min-Guan, Lin, Long-Liu, Wang, Tzu-Fan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309298/ https://www.ncbi.nlm.nih.gov/pubmed/25667926 http://dx.doi.org/10.1155/2015/806847 |
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