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Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy

Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonspherici...

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Autores principales: Serebrennikova, Yulia M., Huffman, Debra E., Garcia-Rubio, Luis H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309305/
https://www.ncbi.nlm.nih.gov/pubmed/25654099
http://dx.doi.org/10.1155/2015/382641
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author Serebrennikova, Yulia M.
Huffman, Debra E.
Garcia-Rubio, Luis H.
author_facet Serebrennikova, Yulia M.
Huffman, Debra E.
Garcia-Rubio, Luis H.
author_sort Serebrennikova, Yulia M.
collection PubMed
description Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs. The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies. When MWT performance was evaluated against a standard reference system, very good agreement between two methods, with R (2) > 0.85 for all indices studied, was demonstrated. The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity. The MWT spectra of RBCs infected with malaria parasite Plasmodium falciparum at different life cycle stages were analyzed for RBC morphological parameters. The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions. The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states.
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spelling pubmed-43093052015-02-04 Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy Serebrennikova, Yulia M. Huffman, Debra E. Garcia-Rubio, Luis H. Biomed Res Int Review Article Multiwavelength transmission (MWT) spectroscopy was applied to the investigation of the morphological parameters and composition of red blood cells (RBCs). The MWT spectra were quantitatively analyzed with a Mie theory based interpretation model modified to incorporate the effects of the nonsphericity and orientation of RBCs. The MWT spectra of the healthy and anemic samples were investigated for the RBC indices in open and blinded studies. When MWT performance was evaluated against a standard reference system, very good agreement between two methods, with R (2) > 0.85 for all indices studied, was demonstrated. The RBC morphological parameters were used to characterize three types of anemia and to draw an association between RBC morphology and anemia severity. The MWT spectra of RBCs infected with malaria parasite Plasmodium falciparum at different life cycle stages were analyzed for RBC morphological parameters. The changes in the RBC volume, surface area, aspect ratio, and hemoglobin composition were used to trace the morphological and compositional alterations in the infected RBCs occurring with parasites' development and to provide insights into parasite-host interactions. The MWT method was shown to be reliable for determination of the RBC morphological parameters and to be valuable for identification of the RBC pathologic changes and disease states. Hindawi Publishing Corporation 2015 2015-01-12 /pmc/articles/PMC4309305/ /pubmed/25654099 http://dx.doi.org/10.1155/2015/382641 Text en Copyright © 2015 Yulia M. Serebrennikova et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Serebrennikova, Yulia M.
Huffman, Debra E.
Garcia-Rubio, Luis H.
Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy
title Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy
title_full Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy
title_fullStr Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy
title_full_unstemmed Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy
title_short Characterization of Red Blood Cells with Multiwavelength Transmission Spectroscopy
title_sort characterization of red blood cells with multiwavelength transmission spectroscopy
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309305/
https://www.ncbi.nlm.nih.gov/pubmed/25654099
http://dx.doi.org/10.1155/2015/382641
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