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RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures

We propose a human-derived neuro-/glial cell three-dimensional in vitro model to investigate the effects of microgravity on cell-cell interactions. A rotary cell-culture system (RCCS) bioreactor was used to generate a modelled microgravity environment, and morphofunctional features of glial-like GL1...

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Autores principales: Morabito, Caterina, Steimberg, Nathalie, Mazzoleni, Giovanna, Guarnieri, Simone, Fanò-Illic, Giorgio, Mariggiò, Maria A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309310/
https://www.ncbi.nlm.nih.gov/pubmed/25654124
http://dx.doi.org/10.1155/2015/754283
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author Morabito, Caterina
Steimberg, Nathalie
Mazzoleni, Giovanna
Guarnieri, Simone
Fanò-Illic, Giorgio
Mariggiò, Maria A.
author_facet Morabito, Caterina
Steimberg, Nathalie
Mazzoleni, Giovanna
Guarnieri, Simone
Fanò-Illic, Giorgio
Mariggiò, Maria A.
author_sort Morabito, Caterina
collection PubMed
description We propose a human-derived neuro-/glial cell three-dimensional in vitro model to investigate the effects of microgravity on cell-cell interactions. A rotary cell-culture system (RCCS) bioreactor was used to generate a modelled microgravity environment, and morphofunctional features of glial-like GL15 and neuronal-like SH-SY5Y cells in three-dimensional individual cultures (monotypic aggregates) and cocultures (heterotypic aggregates) were analysed. Cell survival was maintained within all cell aggregates over 2 weeks of culture. Moreover, compared to cells as traditional static monolayers, cell aggregates cultured under modelled microgravity showed increased expression of specific differentiation markers (e.g., GL15 cells: GFAP, S100B; SH-SY5Y cells: GAP43) and modulation of functional cell-cell interactions (e.g., N-CAM and Cx43 expression and localisation). In conclusion, this culture model opens a wide range of specific investigations at the molecular, biochemical, and morphological levels, and it represents an important tool for in vitro studies into dynamic interactions and responses of nervous system cell components to microgravity environmental conditions.
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spelling pubmed-43093102015-02-04 RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures Morabito, Caterina Steimberg, Nathalie Mazzoleni, Giovanna Guarnieri, Simone Fanò-Illic, Giorgio Mariggiò, Maria A. Biomed Res Int Research Article We propose a human-derived neuro-/glial cell three-dimensional in vitro model to investigate the effects of microgravity on cell-cell interactions. A rotary cell-culture system (RCCS) bioreactor was used to generate a modelled microgravity environment, and morphofunctional features of glial-like GL15 and neuronal-like SH-SY5Y cells in three-dimensional individual cultures (monotypic aggregates) and cocultures (heterotypic aggregates) were analysed. Cell survival was maintained within all cell aggregates over 2 weeks of culture. Moreover, compared to cells as traditional static monolayers, cell aggregates cultured under modelled microgravity showed increased expression of specific differentiation markers (e.g., GL15 cells: GFAP, S100B; SH-SY5Y cells: GAP43) and modulation of functional cell-cell interactions (e.g., N-CAM and Cx43 expression and localisation). In conclusion, this culture model opens a wide range of specific investigations at the molecular, biochemical, and morphological levels, and it represents an important tool for in vitro studies into dynamic interactions and responses of nervous system cell components to microgravity environmental conditions. Hindawi Publishing Corporation 2015 2015-01-13 /pmc/articles/PMC4309310/ /pubmed/25654124 http://dx.doi.org/10.1155/2015/754283 Text en Copyright © 2015 Caterina Morabito et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Morabito, Caterina
Steimberg, Nathalie
Mazzoleni, Giovanna
Guarnieri, Simone
Fanò-Illic, Giorgio
Mariggiò, Maria A.
RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures
title RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures
title_full RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures
title_fullStr RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures
title_full_unstemmed RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures
title_short RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures
title_sort rccs bioreactor-based modelled microgravity induces significant changes on in vitro 3d neuroglial cell cultures
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309310/
https://www.ncbi.nlm.nih.gov/pubmed/25654124
http://dx.doi.org/10.1155/2015/754283
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