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Capacitance-modulated transistor detects odorant binding protein chiral interactions

Peripheral events in olfaction involve odorant binding proteins (OBPs) whose role in the recognition of different volatile chemicals is yet unclear. Here we report on the sensitive and quantitative measurement of the weak interactions associated with neutral enantiomers differentially binding to OBP...

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Autores principales: Mulla, Mohammad Yusuf, Tuccori, Elena, Magliulo, Maria, Lattanzi, Gianluca, Palazzo, Gerardo, Persaud, Krishna, Torsi, Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309438/
https://www.ncbi.nlm.nih.gov/pubmed/25591754
http://dx.doi.org/10.1038/ncomms7010
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author Mulla, Mohammad Yusuf
Tuccori, Elena
Magliulo, Maria
Lattanzi, Gianluca
Palazzo, Gerardo
Persaud, Krishna
Torsi, Luisa
author_facet Mulla, Mohammad Yusuf
Tuccori, Elena
Magliulo, Maria
Lattanzi, Gianluca
Palazzo, Gerardo
Persaud, Krishna
Torsi, Luisa
author_sort Mulla, Mohammad Yusuf
collection PubMed
description Peripheral events in olfaction involve odorant binding proteins (OBPs) whose role in the recognition of different volatile chemicals is yet unclear. Here we report on the sensitive and quantitative measurement of the weak interactions associated with neutral enantiomers differentially binding to OBPs immobilized through a self-assembled monolayer to the gate of an organic bio-electronic transistor. The transduction is remarkably sensitive as the transistor output current is governed by the small capacitance of the protein layer undergoing minute changes as the ligand–protein complex is formed. Accurate determination of the free-energy balances and of the capacitance changes associated with the binding process allows derivation of the free-energy components as well as of the occurrence of conformational events associated with OBP ligand binding. Capacitance-modulated transistors open a new pathway for the study of ultra-weak molecular interactions in surface-bound protein–ligand complexes through an approach that combines bio-chemical and electronic thermodynamic parameters.
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spelling pubmed-43094382015-02-09 Capacitance-modulated transistor detects odorant binding protein chiral interactions Mulla, Mohammad Yusuf Tuccori, Elena Magliulo, Maria Lattanzi, Gianluca Palazzo, Gerardo Persaud, Krishna Torsi, Luisa Nat Commun Article Peripheral events in olfaction involve odorant binding proteins (OBPs) whose role in the recognition of different volatile chemicals is yet unclear. Here we report on the sensitive and quantitative measurement of the weak interactions associated with neutral enantiomers differentially binding to OBPs immobilized through a self-assembled monolayer to the gate of an organic bio-electronic transistor. The transduction is remarkably sensitive as the transistor output current is governed by the small capacitance of the protein layer undergoing minute changes as the ligand–protein complex is formed. Accurate determination of the free-energy balances and of the capacitance changes associated with the binding process allows derivation of the free-energy components as well as of the occurrence of conformational events associated with OBP ligand binding. Capacitance-modulated transistors open a new pathway for the study of ultra-weak molecular interactions in surface-bound protein–ligand complexes through an approach that combines bio-chemical and electronic thermodynamic parameters. Nature Pub. Group 2015-01-16 /pmc/articles/PMC4309438/ /pubmed/25591754 http://dx.doi.org/10.1038/ncomms7010 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mulla, Mohammad Yusuf
Tuccori, Elena
Magliulo, Maria
Lattanzi, Gianluca
Palazzo, Gerardo
Persaud, Krishna
Torsi, Luisa
Capacitance-modulated transistor detects odorant binding protein chiral interactions
title Capacitance-modulated transistor detects odorant binding protein chiral interactions
title_full Capacitance-modulated transistor detects odorant binding protein chiral interactions
title_fullStr Capacitance-modulated transistor detects odorant binding protein chiral interactions
title_full_unstemmed Capacitance-modulated transistor detects odorant binding protein chiral interactions
title_short Capacitance-modulated transistor detects odorant binding protein chiral interactions
title_sort capacitance-modulated transistor detects odorant binding protein chiral interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309438/
https://www.ncbi.nlm.nih.gov/pubmed/25591754
http://dx.doi.org/10.1038/ncomms7010
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