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Elastin aging and lipid oxidation products in human aorta
Vascular aging is associated with structural and functional modifications of the arteries, and by an increase in arterial wall thickening in the intima and the media, mainly resulting from structural modifications of the extracellular matrix (ECM) components. Among the factors known to accumulate wi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309857/ https://www.ncbi.nlm.nih.gov/pubmed/25553420 http://dx.doi.org/10.1016/j.redox.2014.12.008 |
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author | Zarkovic, Kamelija Larroque-Cardoso, Pauline Pucelle, Mélanie Salvayre, Robert Waeg, Georg Nègre-Salvayre, Anne Zarkovic, Neven |
author_facet | Zarkovic, Kamelija Larroque-Cardoso, Pauline Pucelle, Mélanie Salvayre, Robert Waeg, Georg Nègre-Salvayre, Anne Zarkovic, Neven |
author_sort | Zarkovic, Kamelija |
collection | PubMed |
description | Vascular aging is associated with structural and functional modifications of the arteries, and by an increase in arterial wall thickening in the intima and the media, mainly resulting from structural modifications of the extracellular matrix (ECM) components. Among the factors known to accumulate with aging, advanced lipid peroxidation end products (ALEs) are a hallmark of oxidative stress-associated diseases such as atherosclerosis. Aldehydes generated from the peroxidation of polyunsaturated fatty acids (PUFA), (4-hydroxynonenal, malondialdehyde, acrolein), form adducts on cellular proteins, leading to a progressive protein dysfunction with consequences in the pathophysiology of vascular aging. The contribution of these aldehydes to ECM modification is not known. This study was carried out to investigate whether aldehyde-adducts are detected in the intima and media in human aorta, whether their level is increased in vascular aging, and whether elastin fibers are a target of aldehyde-adduct formation. Immunohistological and confocal immunofluorescence studies indicate that 4-HNE-histidine-adducts accumulate in an age-related manner in the intima, media and adventitia layers of human aortas, and are mainly expressed in smooth muscle cells. In contrast, even if the structure of elastin fiber is strongly altered in the aged vessels, our results show that elastin is not or very poorly modified by 4-HNE. These data indicate a complex role for lipid peroxidation and in particular for 4-HNE in elastin homeostasis, in the vascular wall remodeling during aging and atherosclerosis development. |
format | Online Article Text |
id | pubmed-4309857 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-43098572015-02-14 Elastin aging and lipid oxidation products in human aorta Zarkovic, Kamelija Larroque-Cardoso, Pauline Pucelle, Mélanie Salvayre, Robert Waeg, Georg Nègre-Salvayre, Anne Zarkovic, Neven Redox Biol Research Paper Vascular aging is associated with structural and functional modifications of the arteries, and by an increase in arterial wall thickening in the intima and the media, mainly resulting from structural modifications of the extracellular matrix (ECM) components. Among the factors known to accumulate with aging, advanced lipid peroxidation end products (ALEs) are a hallmark of oxidative stress-associated diseases such as atherosclerosis. Aldehydes generated from the peroxidation of polyunsaturated fatty acids (PUFA), (4-hydroxynonenal, malondialdehyde, acrolein), form adducts on cellular proteins, leading to a progressive protein dysfunction with consequences in the pathophysiology of vascular aging. The contribution of these aldehydes to ECM modification is not known. This study was carried out to investigate whether aldehyde-adducts are detected in the intima and media in human aorta, whether their level is increased in vascular aging, and whether elastin fibers are a target of aldehyde-adduct formation. Immunohistological and confocal immunofluorescence studies indicate that 4-HNE-histidine-adducts accumulate in an age-related manner in the intima, media and adventitia layers of human aortas, and are mainly expressed in smooth muscle cells. In contrast, even if the structure of elastin fiber is strongly altered in the aged vessels, our results show that elastin is not or very poorly modified by 4-HNE. These data indicate a complex role for lipid peroxidation and in particular for 4-HNE in elastin homeostasis, in the vascular wall remodeling during aging and atherosclerosis development. Elsevier 2014-12-18 /pmc/articles/PMC4309857/ /pubmed/25553420 http://dx.doi.org/10.1016/j.redox.2014.12.008 Text en © 2014 Published by Elsevier B.V. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Research Paper Zarkovic, Kamelija Larroque-Cardoso, Pauline Pucelle, Mélanie Salvayre, Robert Waeg, Georg Nègre-Salvayre, Anne Zarkovic, Neven Elastin aging and lipid oxidation products in human aorta |
title | Elastin aging and lipid oxidation products in human aorta |
title_full | Elastin aging and lipid oxidation products in human aorta |
title_fullStr | Elastin aging and lipid oxidation products in human aorta |
title_full_unstemmed | Elastin aging and lipid oxidation products in human aorta |
title_short | Elastin aging and lipid oxidation products in human aorta |
title_sort | elastin aging and lipid oxidation products in human aorta |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309857/ https://www.ncbi.nlm.nih.gov/pubmed/25553420 http://dx.doi.org/10.1016/j.redox.2014.12.008 |
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