Single tube tetraplex PCR based screening of a SNP at exon 14 region of bovine ITGB6 among different Zebu breeds

The present study was aimed to screen genetic variation within a SNP (rs136500299) located at exon 14 region of bovine ITGB6 gene among different Zebu cattle breeds. The genotyping method describe in the present study is a tetraplex ARMS PCR, which offers extremely fast, economical, and simple detec...

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Autores principales: Singh, Rani, Deb, Rajib, Singh, Umesh, Raja, T.V., Alex, Rani, Kumar, Sushil, Chakraborti, Soumendu, Sengar, Gyanendra, Sharma, Sheetal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310182/
https://www.ncbi.nlm.nih.gov/pubmed/25685717
http://dx.doi.org/10.1016/j.mgene.2014.12.004
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author Singh, Rani
Deb, Rajib
Singh, Umesh
Raja, T.V.
Alex, Rani
Kumar, Sushil
Chakraborti, Soumendu
Sengar, Gyanendra
Sharma, Sheetal
author_facet Singh, Rani
Deb, Rajib
Singh, Umesh
Raja, T.V.
Alex, Rani
Kumar, Sushil
Chakraborti, Soumendu
Sengar, Gyanendra
Sharma, Sheetal
author_sort Singh, Rani
collection PubMed
description The present study was aimed to screen genetic variation within a SNP (rs136500299) located at exon 14 region of bovine ITGB6 gene among different Zebu cattle breeds. The genotyping method describe in the present study is a tetraplex ARMS PCR, which offers extremely fast, economical, and simple detection tool. The distribution of the ITGB6 genotypes among the different breeds studied suggested that the populations were under Hardy–Weinberg equilibrium. Our findings revealed that TT genotypes are widely distributed among different Zebu cattle breeds, which can be associated with the resistance to FMD virus, as the Bos indicus are more resistant to FMD virus in comparison to Bos taurus.
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spelling pubmed-43101822015-02-14 Single tube tetraplex PCR based screening of a SNP at exon 14 region of bovine ITGB6 among different Zebu breeds Singh, Rani Deb, Rajib Singh, Umesh Raja, T.V. Alex, Rani Kumar, Sushil Chakraborti, Soumendu Sengar, Gyanendra Sharma, Sheetal Meta Gene Article The present study was aimed to screen genetic variation within a SNP (rs136500299) located at exon 14 region of bovine ITGB6 gene among different Zebu cattle breeds. The genotyping method describe in the present study is a tetraplex ARMS PCR, which offers extremely fast, economical, and simple detection tool. The distribution of the ITGB6 genotypes among the different breeds studied suggested that the populations were under Hardy–Weinberg equilibrium. Our findings revealed that TT genotypes are widely distributed among different Zebu cattle breeds, which can be associated with the resistance to FMD virus, as the Bos indicus are more resistant to FMD virus in comparison to Bos taurus. Elsevier 2015-01-10 /pmc/articles/PMC4310182/ /pubmed/25685717 http://dx.doi.org/10.1016/j.mgene.2014.12.004 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Singh, Rani
Deb, Rajib
Singh, Umesh
Raja, T.V.
Alex, Rani
Kumar, Sushil
Chakraborti, Soumendu
Sengar, Gyanendra
Sharma, Sheetal
Single tube tetraplex PCR based screening of a SNP at exon 14 region of bovine ITGB6 among different Zebu breeds
title Single tube tetraplex PCR based screening of a SNP at exon 14 region of bovine ITGB6 among different Zebu breeds
title_full Single tube tetraplex PCR based screening of a SNP at exon 14 region of bovine ITGB6 among different Zebu breeds
title_fullStr Single tube tetraplex PCR based screening of a SNP at exon 14 region of bovine ITGB6 among different Zebu breeds
title_full_unstemmed Single tube tetraplex PCR based screening of a SNP at exon 14 region of bovine ITGB6 among different Zebu breeds
title_short Single tube tetraplex PCR based screening of a SNP at exon 14 region of bovine ITGB6 among different Zebu breeds
title_sort single tube tetraplex pcr based screening of a snp at exon 14 region of bovine itgb6 among different zebu breeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310182/
https://www.ncbi.nlm.nih.gov/pubmed/25685717
http://dx.doi.org/10.1016/j.mgene.2014.12.004
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