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Blunted Activation of Rho-Kinase in Yak Pulmonary Circulation
Yaks have adapted to high altitude and they do not develop hypoxic pulmonary hypertension. Although we previously identified the important role of augmented nitric oxide synthase activity in the yak pulmonary circulatory system, evidence of the direct involvement of Rho-kinase as a basal vascular to...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310305/ https://www.ncbi.nlm.nih.gov/pubmed/25654121 http://dx.doi.org/10.1155/2015/720250 |
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author | Ishizaki, Takeshi Mizuno, Shiro Sakai, Akio Matsukawa, Shigeru Kojonazarov, Baktybek Zamirbek, Baiserkeev Umeda, Yukihiro Morikawa, Miwa Anzai, Masaki Ishizuka, Tamotsu Aldashev, Almaz |
author_facet | Ishizaki, Takeshi Mizuno, Shiro Sakai, Akio Matsukawa, Shigeru Kojonazarov, Baktybek Zamirbek, Baiserkeev Umeda, Yukihiro Morikawa, Miwa Anzai, Masaki Ishizuka, Tamotsu Aldashev, Almaz |
author_sort | Ishizaki, Takeshi |
collection | PubMed |
description | Yaks have adapted to high altitude and they do not develop hypoxic pulmonary hypertension. Although we previously identified the important role of augmented nitric oxide synthase activity in the yak pulmonary circulatory system, evidence of the direct involvement of Rho-kinase as a basal vascular tone regulator is lacking. Four domesticated male pure-bred yaks and four bulls that were born and raised at an altitude of 3000 m in the Tien-Shan mountains were studied at an altitude of 3,100 m. Mean pulmonary artery pressure (mPAP) was measured before and after fasudil (60 mg in 20 mL of saline) was intravenously administered using a Swan-Ganz catheter at a rate of 3.3 mL/min for 30 min. Fasudil decreased mPAP in bulls from 67.8±14.9 to 32.3±5.3 mmHg (P < 0.05) after 15 min and the level was maintained for 30 min, but it merely blunted mPAP in yaks from 28.2±4.5 to 25.1±11.1 and 23.2±2.7 mmHg after 5 and 30 min, respectively. These findings comprise the first evidence of a modest role of Rho-kinase in the maintenance of pulmonary artery pressure in the yak. |
format | Online Article Text |
id | pubmed-4310305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43103052015-02-04 Blunted Activation of Rho-Kinase in Yak Pulmonary Circulation Ishizaki, Takeshi Mizuno, Shiro Sakai, Akio Matsukawa, Shigeru Kojonazarov, Baktybek Zamirbek, Baiserkeev Umeda, Yukihiro Morikawa, Miwa Anzai, Masaki Ishizuka, Tamotsu Aldashev, Almaz Biomed Res Int Research Article Yaks have adapted to high altitude and they do not develop hypoxic pulmonary hypertension. Although we previously identified the important role of augmented nitric oxide synthase activity in the yak pulmonary circulatory system, evidence of the direct involvement of Rho-kinase as a basal vascular tone regulator is lacking. Four domesticated male pure-bred yaks and four bulls that were born and raised at an altitude of 3000 m in the Tien-Shan mountains were studied at an altitude of 3,100 m. Mean pulmonary artery pressure (mPAP) was measured before and after fasudil (60 mg in 20 mL of saline) was intravenously administered using a Swan-Ganz catheter at a rate of 3.3 mL/min for 30 min. Fasudil decreased mPAP in bulls from 67.8±14.9 to 32.3±5.3 mmHg (P < 0.05) after 15 min and the level was maintained for 30 min, but it merely blunted mPAP in yaks from 28.2±4.5 to 25.1±11.1 and 23.2±2.7 mmHg after 5 and 30 min, respectively. These findings comprise the first evidence of a modest role of Rho-kinase in the maintenance of pulmonary artery pressure in the yak. Hindawi Publishing Corporation 2015 2015-01-14 /pmc/articles/PMC4310305/ /pubmed/25654121 http://dx.doi.org/10.1155/2015/720250 Text en Copyright © 2015 Takeshi Ishizaki et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ishizaki, Takeshi Mizuno, Shiro Sakai, Akio Matsukawa, Shigeru Kojonazarov, Baktybek Zamirbek, Baiserkeev Umeda, Yukihiro Morikawa, Miwa Anzai, Masaki Ishizuka, Tamotsu Aldashev, Almaz Blunted Activation of Rho-Kinase in Yak Pulmonary Circulation |
title | Blunted Activation of Rho-Kinase in Yak Pulmonary Circulation |
title_full | Blunted Activation of Rho-Kinase in Yak Pulmonary Circulation |
title_fullStr | Blunted Activation of Rho-Kinase in Yak Pulmonary Circulation |
title_full_unstemmed | Blunted Activation of Rho-Kinase in Yak Pulmonary Circulation |
title_short | Blunted Activation of Rho-Kinase in Yak Pulmonary Circulation |
title_sort | blunted activation of rho-kinase in yak pulmonary circulation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310305/ https://www.ncbi.nlm.nih.gov/pubmed/25654121 http://dx.doi.org/10.1155/2015/720250 |
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