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Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum) on Eimeria papillata-Induced Infection in Mice
Coccidiosis is the most prevalent disease causing widespread economic loss, especially in poultry farms. Here, we investigated the effects of pomegranate peel extract (PPE) on the outcome of coccidiosis caused by Eimeria papillata in mice. The data showed that mice infected with E. papillata and tre...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310320/ https://www.ncbi.nlm.nih.gov/pubmed/25654088 http://dx.doi.org/10.1155/2015/219670 |
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author | Amer, Omar S. O. Dkhil, Mohamed A. Hikal, Wafaa M. Al-Quraishy, Saleh |
author_facet | Amer, Omar S. O. Dkhil, Mohamed A. Hikal, Wafaa M. Al-Quraishy, Saleh |
author_sort | Amer, Omar S. O. |
collection | PubMed |
description | Coccidiosis is the most prevalent disease causing widespread economic loss, especially in poultry farms. Here, we investigated the effects of pomegranate peel extract (PPE) on the outcome of coccidiosis caused by Eimeria papillata in mice. The data showed that mice infected with E. papillata and treated with PPE revealed a significant decrease in the output of oocysts in their faeces by day 5 p.i. Infection also induced inflammation and injury of the jejunum. This was evidenced (i) as increases in reactive oxygen species, (ii), as increased neutrophils and decreased lymphocytes in blood (ii) as increased mRNA levels of inducible nitric oxide synthase (iNOS), Bcl-2 gene, and of the cytokines interferon gamma (IFN-γ), tumour necrosis factor-α (TNF-α), and interleukin-1β (IL-1β), and (iv) as downregulation of mucin gene MUC2 mRNA. All these infection-induced parameters were significantly altered during PPE treatment. In particular, PPE counteracted the E. papillata-induced loss of the total antioxidant capacity. Our data indicated that PPE treatment significantly attenuated inflammation and injury of the jejunum induced by E. papillata infections. |
format | Online Article Text |
id | pubmed-4310320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43103202015-02-04 Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum) on Eimeria papillata-Induced Infection in Mice Amer, Omar S. O. Dkhil, Mohamed A. Hikal, Wafaa M. Al-Quraishy, Saleh Biomed Res Int Research Article Coccidiosis is the most prevalent disease causing widespread economic loss, especially in poultry farms. Here, we investigated the effects of pomegranate peel extract (PPE) on the outcome of coccidiosis caused by Eimeria papillata in mice. The data showed that mice infected with E. papillata and treated with PPE revealed a significant decrease in the output of oocysts in their faeces by day 5 p.i. Infection also induced inflammation and injury of the jejunum. This was evidenced (i) as increases in reactive oxygen species, (ii), as increased neutrophils and decreased lymphocytes in blood (ii) as increased mRNA levels of inducible nitric oxide synthase (iNOS), Bcl-2 gene, and of the cytokines interferon gamma (IFN-γ), tumour necrosis factor-α (TNF-α), and interleukin-1β (IL-1β), and (iv) as downregulation of mucin gene MUC2 mRNA. All these infection-induced parameters were significantly altered during PPE treatment. In particular, PPE counteracted the E. papillata-induced loss of the total antioxidant capacity. Our data indicated that PPE treatment significantly attenuated inflammation and injury of the jejunum induced by E. papillata infections. Hindawi Publishing Corporation 2015 2015-01-15 /pmc/articles/PMC4310320/ /pubmed/25654088 http://dx.doi.org/10.1155/2015/219670 Text en Copyright © 2015 Omar S. O. Amer et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Amer, Omar S. O. Dkhil, Mohamed A. Hikal, Wafaa M. Al-Quraishy, Saleh Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum) on Eimeria papillata-Induced Infection in Mice |
title | Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum) on Eimeria papillata-Induced Infection in Mice |
title_full | Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum) on Eimeria papillata-Induced Infection in Mice |
title_fullStr | Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum) on Eimeria papillata-Induced Infection in Mice |
title_full_unstemmed | Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum) on Eimeria papillata-Induced Infection in Mice |
title_short | Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum) on Eimeria papillata-Induced Infection in Mice |
title_sort | antioxidant and anti-inflammatory activities of pomegranate (punica granatum) on eimeria papillata-induced infection in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310320/ https://www.ncbi.nlm.nih.gov/pubmed/25654088 http://dx.doi.org/10.1155/2015/219670 |
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