Cargando…
Pien Tze Huang Inhibits Hypoxia-Induced Angiogenesis via HIF-1α/VEGF-A Pathway in Colorectal Cancer
Hypoxia-induced angiogenesis plays an important role in the development and metastasis of solid tumors and is highly regulated by HIF-1α/VEGF-A pathway. Therefore, inhibiting tumor angiogenesis via suppression of HIF-1α/VEGF-A signaling represents a promising strategy for anticancer treatment. As a...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310443/ https://www.ncbi.nlm.nih.gov/pubmed/25649293 http://dx.doi.org/10.1155/2015/454279 |
_version_ | 1782354877443211264 |
---|---|
author | Chen, Hongwei Feng, Jianyu Zhang, Yuchen Shen, Aling Chen, Youqin Lin, Jiumao Lin, Wei Sferra, Thomas J. Peng, Jun |
author_facet | Chen, Hongwei Feng, Jianyu Zhang, Yuchen Shen, Aling Chen, Youqin Lin, Jiumao Lin, Wei Sferra, Thomas J. Peng, Jun |
author_sort | Chen, Hongwei |
collection | PubMed |
description | Hypoxia-induced angiogenesis plays an important role in the development and metastasis of solid tumors and is highly regulated by HIF-1α/VEGF-A pathway. Therefore, inhibiting tumor angiogenesis via suppression of HIF-1α/VEGF-A signaling represents a promising strategy for anticancer treatment. As a traditional Chinese medicine formula, Pien Tze Huang (PZH) has long been used as a folk remedy for cancer in China and Southeast Asia. Previously, we reported that PZH inhibits colorectal cancer (CRC) growth both in vivo and in vitro. To elucidate the antitumor mechanisms of PZH, in the present study we used human umbilical vein endothelial cells (HUVEC) and colorectal carcinoma HCT-8 cells to evaluate the effects of PZH on hypoxia-induced angiogenesis and investigated the underlying molecular mechanisms. We found that PZH could inhibit hypoxia-induced migration and tube formation of HUVEC cells in a dose-dependent manner, although the low concentrations of PZH had no effect on HUVEC viability. Moreover, PZH inhibited hypoxia-induced activation of HIF-1α signaling and the expression of VEGF-A and/or VEGFR2 in both HCT-8 and HUVEC cells. Collectively, our findings suggest that PZH can inhibit hypoxia-induced tumor angiogenesis via suppression of HIF-1α/VEGF-A pathway. |
format | Online Article Text |
id | pubmed-4310443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43104432015-02-03 Pien Tze Huang Inhibits Hypoxia-Induced Angiogenesis via HIF-1α/VEGF-A Pathway in Colorectal Cancer Chen, Hongwei Feng, Jianyu Zhang, Yuchen Shen, Aling Chen, Youqin Lin, Jiumao Lin, Wei Sferra, Thomas J. Peng, Jun Evid Based Complement Alternat Med Research Article Hypoxia-induced angiogenesis plays an important role in the development and metastasis of solid tumors and is highly regulated by HIF-1α/VEGF-A pathway. Therefore, inhibiting tumor angiogenesis via suppression of HIF-1α/VEGF-A signaling represents a promising strategy for anticancer treatment. As a traditional Chinese medicine formula, Pien Tze Huang (PZH) has long been used as a folk remedy for cancer in China and Southeast Asia. Previously, we reported that PZH inhibits colorectal cancer (CRC) growth both in vivo and in vitro. To elucidate the antitumor mechanisms of PZH, in the present study we used human umbilical vein endothelial cells (HUVEC) and colorectal carcinoma HCT-8 cells to evaluate the effects of PZH on hypoxia-induced angiogenesis and investigated the underlying molecular mechanisms. We found that PZH could inhibit hypoxia-induced migration and tube formation of HUVEC cells in a dose-dependent manner, although the low concentrations of PZH had no effect on HUVEC viability. Moreover, PZH inhibited hypoxia-induced activation of HIF-1α signaling and the expression of VEGF-A and/or VEGFR2 in both HCT-8 and HUVEC cells. Collectively, our findings suggest that PZH can inhibit hypoxia-induced tumor angiogenesis via suppression of HIF-1α/VEGF-A pathway. Hindawi Publishing Corporation 2015 2015-01-14 /pmc/articles/PMC4310443/ /pubmed/25649293 http://dx.doi.org/10.1155/2015/454279 Text en Copyright © 2015 Hongwei Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Hongwei Feng, Jianyu Zhang, Yuchen Shen, Aling Chen, Youqin Lin, Jiumao Lin, Wei Sferra, Thomas J. Peng, Jun Pien Tze Huang Inhibits Hypoxia-Induced Angiogenesis via HIF-1α/VEGF-A Pathway in Colorectal Cancer |
title | Pien Tze Huang Inhibits Hypoxia-Induced Angiogenesis via HIF-1α/VEGF-A Pathway in Colorectal Cancer |
title_full | Pien Tze Huang Inhibits Hypoxia-Induced Angiogenesis via HIF-1α/VEGF-A Pathway in Colorectal Cancer |
title_fullStr | Pien Tze Huang Inhibits Hypoxia-Induced Angiogenesis via HIF-1α/VEGF-A Pathway in Colorectal Cancer |
title_full_unstemmed | Pien Tze Huang Inhibits Hypoxia-Induced Angiogenesis via HIF-1α/VEGF-A Pathway in Colorectal Cancer |
title_short | Pien Tze Huang Inhibits Hypoxia-Induced Angiogenesis via HIF-1α/VEGF-A Pathway in Colorectal Cancer |
title_sort | pien tze huang inhibits hypoxia-induced angiogenesis via hif-1α/vegf-a pathway in colorectal cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310443/ https://www.ncbi.nlm.nih.gov/pubmed/25649293 http://dx.doi.org/10.1155/2015/454279 |
work_keys_str_mv | AT chenhongwei pientzehuanginhibitshypoxiainducedangiogenesisviahif1avegfapathwayincolorectalcancer AT fengjianyu pientzehuanginhibitshypoxiainducedangiogenesisviahif1avegfapathwayincolorectalcancer AT zhangyuchen pientzehuanginhibitshypoxiainducedangiogenesisviahif1avegfapathwayincolorectalcancer AT shenaling pientzehuanginhibitshypoxiainducedangiogenesisviahif1avegfapathwayincolorectalcancer AT chenyouqin pientzehuanginhibitshypoxiainducedangiogenesisviahif1avegfapathwayincolorectalcancer AT linjiumao pientzehuanginhibitshypoxiainducedangiogenesisviahif1avegfapathwayincolorectalcancer AT linwei pientzehuanginhibitshypoxiainducedangiogenesisviahif1avegfapathwayincolorectalcancer AT sferrathomasj pientzehuanginhibitshypoxiainducedangiogenesisviahif1avegfapathwayincolorectalcancer AT pengjun pientzehuanginhibitshypoxiainducedangiogenesisviahif1avegfapathwayincolorectalcancer |