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Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis

Rho GTPases, activated by guanine nucleotide exchange factors (GEFs), are essential regulators of polarized cell growth, cytokinesis, and many other cellular processes. However, the regulation of Rho-GEFs themselves is not well understood. Rgf3 is an essential GEF for Rho1 GTPase in fission yeast. W...

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Autores principales: Davidson, Reshma, Laporte, Damien, Wu, Jian-Qiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310737/
https://www.ncbi.nlm.nih.gov/pubmed/25473118
http://dx.doi.org/10.1091/mbc.E14-07-1252
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author Davidson, Reshma
Laporte, Damien
Wu, Jian-Qiu
author_facet Davidson, Reshma
Laporte, Damien
Wu, Jian-Qiu
author_sort Davidson, Reshma
collection PubMed
description Rho GTPases, activated by guanine nucleotide exchange factors (GEFs), are essential regulators of polarized cell growth, cytokinesis, and many other cellular processes. However, the regulation of Rho-GEFs themselves is not well understood. Rgf3 is an essential GEF for Rho1 GTPase in fission yeast. We show that Rgf3 protein levels and localization are regulated by arrestin-related protein Art1. art1∆ cells lyse during cell separation with a thinner and defective septum. As does Rgf3, Art1 concentrates to the contractile ring starting at early anaphase and spreads to the septum during and after ring constriction. Art1 localization depends on its C-terminus, and Art1 is important for maintaining Rgf3 protein levels. Biochemical experiments reveal that the Rgf3 C-terminus binds to Art1. Using an Rgf3 conditional mutant and mislocalization experiments, we found that Art1 and Rgf3 are interdependent for localization to the division site. As expected, active Rho1 levels at the division site are reduced in art1∆ and rgf3 mutant cells. Taken together, these data reveal that the arrestin family protein Art1 regulates the protein levels and localization of the Rho-GEF Rgf3, which in turn modulates active Rho1 levels during fission yeast cytokinesis.
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spelling pubmed-43107372015-04-16 Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis Davidson, Reshma Laporte, Damien Wu, Jian-Qiu Mol Biol Cell Articles Rho GTPases, activated by guanine nucleotide exchange factors (GEFs), are essential regulators of polarized cell growth, cytokinesis, and many other cellular processes. However, the regulation of Rho-GEFs themselves is not well understood. Rgf3 is an essential GEF for Rho1 GTPase in fission yeast. We show that Rgf3 protein levels and localization are regulated by arrestin-related protein Art1. art1∆ cells lyse during cell separation with a thinner and defective septum. As does Rgf3, Art1 concentrates to the contractile ring starting at early anaphase and spreads to the septum during and after ring constriction. Art1 localization depends on its C-terminus, and Art1 is important for maintaining Rgf3 protein levels. Biochemical experiments reveal that the Rgf3 C-terminus binds to Art1. Using an Rgf3 conditional mutant and mislocalization experiments, we found that Art1 and Rgf3 are interdependent for localization to the division site. As expected, active Rho1 levels at the division site are reduced in art1∆ and rgf3 mutant cells. Taken together, these data reveal that the arrestin family protein Art1 regulates the protein levels and localization of the Rho-GEF Rgf3, which in turn modulates active Rho1 levels during fission yeast cytokinesis. The American Society for Cell Biology 2015-02-01 /pmc/articles/PMC4310737/ /pubmed/25473118 http://dx.doi.org/10.1091/mbc.E14-07-1252 Text en © 2015 Davidson et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Davidson, Reshma
Laporte, Damien
Wu, Jian-Qiu
Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis
title Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis
title_full Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis
title_fullStr Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis
title_full_unstemmed Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis
title_short Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis
title_sort regulation of rho-gef rgf3 by the arrestin art1 in fission yeast cytokinesis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310737/
https://www.ncbi.nlm.nih.gov/pubmed/25473118
http://dx.doi.org/10.1091/mbc.E14-07-1252
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