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Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking

The fusion of GLUT4-containing vesicles with the plasma membrane of adipocytes is a key facet of insulin action. This process is mediated by the formation of functional soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) complexes between the plasma membrane t-SNARE complex...

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Detalles Bibliográficos
Autores principales: Sadler, Jessica B. A., Bryant, Nia J., Gould, Gwyn W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310743/
https://www.ncbi.nlm.nih.gov/pubmed/25501368
http://dx.doi.org/10.1091/mbc.E14-09-1368
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author Sadler, Jessica B. A.
Bryant, Nia J.
Gould, Gwyn W.
author_facet Sadler, Jessica B. A.
Bryant, Nia J.
Gould, Gwyn W.
author_sort Sadler, Jessica B. A.
collection PubMed
description The fusion of GLUT4-containing vesicles with the plasma membrane of adipocytes is a key facet of insulin action. This process is mediated by the formation of functional soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) complexes between the plasma membrane t-SNARE complex and the vesicle v-SNARE or VAMP. The t-SNARE complex consists of Syntaxin4 and SNAP23, and whereas many studies identify VAMP2 as the v-SNARE, others suggest that either VAMP3 or VAMP8 may also fulfil this role. Here we characterized the levels of expression, distribution, and association of all the VAMPs expressed in 3T3-L1 adipocytes to provide the first systematic analysis of all members of this protein family for any cell type. Despite our finding that all VAMP isoforms form SDS-resistant SNARE complexes with Syntaxin4/SNAP23 in vitro, a combination of levels of expression (which vary by >30-fold), subcellular distribution, and coimmunoprecipitation analyses lead us to propose that VAMP2 is the major v-SNARE involved in GLUT4 trafficking to the surface of 3T3-L1 adipocytes.
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spelling pubmed-43107432015-04-16 Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking Sadler, Jessica B. A. Bryant, Nia J. Gould, Gwyn W. Mol Biol Cell Articles The fusion of GLUT4-containing vesicles with the plasma membrane of adipocytes is a key facet of insulin action. This process is mediated by the formation of functional soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) complexes between the plasma membrane t-SNARE complex and the vesicle v-SNARE or VAMP. The t-SNARE complex consists of Syntaxin4 and SNAP23, and whereas many studies identify VAMP2 as the v-SNARE, others suggest that either VAMP3 or VAMP8 may also fulfil this role. Here we characterized the levels of expression, distribution, and association of all the VAMPs expressed in 3T3-L1 adipocytes to provide the first systematic analysis of all members of this protein family for any cell type. Despite our finding that all VAMP isoforms form SDS-resistant SNARE complexes with Syntaxin4/SNAP23 in vitro, a combination of levels of expression (which vary by >30-fold), subcellular distribution, and coimmunoprecipitation analyses lead us to propose that VAMP2 is the major v-SNARE involved in GLUT4 trafficking to the surface of 3T3-L1 adipocytes. The American Society for Cell Biology 2015-02-01 /pmc/articles/PMC4310743/ /pubmed/25501368 http://dx.doi.org/10.1091/mbc.E14-09-1368 Text en © 2015 Sadler et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Sadler, Jessica B. A.
Bryant, Nia J.
Gould, Gwyn W.
Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking
title Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking
title_full Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking
title_fullStr Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking
title_full_unstemmed Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking
title_short Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking
title_sort characterization of vamp isoforms in 3t3-l1 adipocytes: implications for glut4 trafficking
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310743/
https://www.ncbi.nlm.nih.gov/pubmed/25501368
http://dx.doi.org/10.1091/mbc.E14-09-1368
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