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Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210
Golgins are extended coiled-coil proteins believed to participate in membrane-tethering events at the Golgi apparatus. However, the importance of golgin-mediated tethering remains poorly defined, and alternative functions for golgins have been proposed. Moreover, although golgins bind to Rab GTPases...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310744/ https://www.ncbi.nlm.nih.gov/pubmed/25473115 http://dx.doi.org/10.1091/mbc.E14-10-1450 |
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author | Sato, Keisuke Roboti, Peristera Mironov, Alexander A. Lowe, Martin |
author_facet | Sato, Keisuke Roboti, Peristera Mironov, Alexander A. Lowe, Martin |
author_sort | Sato, Keisuke |
collection | PubMed |
description | Golgins are extended coiled-coil proteins believed to participate in membrane-tethering events at the Golgi apparatus. However, the importance of golgin-mediated tethering remains poorly defined, and alternative functions for golgins have been proposed. Moreover, although golgins bind to Rab GTPases, the functional significance of Rab binding has yet to be determined. In this study, we show that depletion of the golgin GMAP-210 causes a loss of Golgi cisternae and accumulation of numerous vesicles. GMAP-210 function in vivo is dependent upon its ability to tether membranes, which is mediated exclusively by the amino-terminal ALPS motif. Binding to Rab2 is also important for GMAP-210 function, although it is dispensable for tethering per se. GMAP-210 length is also functionally important in vivo. Together our results indicate a key role for GMAP-210–mediated membrane tethering in maintaining Golgi structure and support a role for Rab2 binding in linking tethering with downstream docking and fusion events at the Golgi apparatus. |
format | Online Article Text |
id | pubmed-4310744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-43107442015-04-16 Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210 Sato, Keisuke Roboti, Peristera Mironov, Alexander A. Lowe, Martin Mol Biol Cell Articles Golgins are extended coiled-coil proteins believed to participate in membrane-tethering events at the Golgi apparatus. However, the importance of golgin-mediated tethering remains poorly defined, and alternative functions for golgins have been proposed. Moreover, although golgins bind to Rab GTPases, the functional significance of Rab binding has yet to be determined. In this study, we show that depletion of the golgin GMAP-210 causes a loss of Golgi cisternae and accumulation of numerous vesicles. GMAP-210 function in vivo is dependent upon its ability to tether membranes, which is mediated exclusively by the amino-terminal ALPS motif. Binding to Rab2 is also important for GMAP-210 function, although it is dispensable for tethering per se. GMAP-210 length is also functionally important in vivo. Together our results indicate a key role for GMAP-210–mediated membrane tethering in maintaining Golgi structure and support a role for Rab2 binding in linking tethering with downstream docking and fusion events at the Golgi apparatus. The American Society for Cell Biology 2015-02-01 /pmc/articles/PMC4310744/ /pubmed/25473115 http://dx.doi.org/10.1091/mbc.E14-10-1450 Text en © 2015 Sato, Roboti, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Sato, Keisuke Roboti, Peristera Mironov, Alexander A. Lowe, Martin Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210 |
title | Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210 |
title_full | Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210 |
title_fullStr | Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210 |
title_full_unstemmed | Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210 |
title_short | Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210 |
title_sort | coupling of vesicle tethering and rab binding is required for in vivo functionality of the golgin gmap-210 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310744/ https://www.ncbi.nlm.nih.gov/pubmed/25473115 http://dx.doi.org/10.1091/mbc.E14-10-1450 |
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