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Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210

Golgins are extended coiled-coil proteins believed to participate in membrane-tethering events at the Golgi apparatus. However, the importance of golgin-mediated tethering remains poorly defined, and alternative functions for golgins have been proposed. Moreover, although golgins bind to Rab GTPases...

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Autores principales: Sato, Keisuke, Roboti, Peristera, Mironov, Alexander A., Lowe, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310744/
https://www.ncbi.nlm.nih.gov/pubmed/25473115
http://dx.doi.org/10.1091/mbc.E14-10-1450
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author Sato, Keisuke
Roboti, Peristera
Mironov, Alexander A.
Lowe, Martin
author_facet Sato, Keisuke
Roboti, Peristera
Mironov, Alexander A.
Lowe, Martin
author_sort Sato, Keisuke
collection PubMed
description Golgins are extended coiled-coil proteins believed to participate in membrane-tethering events at the Golgi apparatus. However, the importance of golgin-mediated tethering remains poorly defined, and alternative functions for golgins have been proposed. Moreover, although golgins bind to Rab GTPases, the functional significance of Rab binding has yet to be determined. In this study, we show that depletion of the golgin GMAP-210 causes a loss of Golgi cisternae and accumulation of numerous vesicles. GMAP-210 function in vivo is dependent upon its ability to tether membranes, which is mediated exclusively by the amino-terminal ALPS motif. Binding to Rab2 is also important for GMAP-210 function, although it is dispensable for tethering per se. GMAP-210 length is also functionally important in vivo. Together our results indicate a key role for GMAP-210–mediated membrane tethering in maintaining Golgi structure and support a role for Rab2 binding in linking tethering with downstream docking and fusion events at the Golgi apparatus.
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spelling pubmed-43107442015-04-16 Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210 Sato, Keisuke Roboti, Peristera Mironov, Alexander A. Lowe, Martin Mol Biol Cell Articles Golgins are extended coiled-coil proteins believed to participate in membrane-tethering events at the Golgi apparatus. However, the importance of golgin-mediated tethering remains poorly defined, and alternative functions for golgins have been proposed. Moreover, although golgins bind to Rab GTPases, the functional significance of Rab binding has yet to be determined. In this study, we show that depletion of the golgin GMAP-210 causes a loss of Golgi cisternae and accumulation of numerous vesicles. GMAP-210 function in vivo is dependent upon its ability to tether membranes, which is mediated exclusively by the amino-terminal ALPS motif. Binding to Rab2 is also important for GMAP-210 function, although it is dispensable for tethering per se. GMAP-210 length is also functionally important in vivo. Together our results indicate a key role for GMAP-210–mediated membrane tethering in maintaining Golgi structure and support a role for Rab2 binding in linking tethering with downstream docking and fusion events at the Golgi apparatus. The American Society for Cell Biology 2015-02-01 /pmc/articles/PMC4310744/ /pubmed/25473115 http://dx.doi.org/10.1091/mbc.E14-10-1450 Text en © 2015 Sato, Roboti, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Sato, Keisuke
Roboti, Peristera
Mironov, Alexander A.
Lowe, Martin
Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210
title Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210
title_full Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210
title_fullStr Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210
title_full_unstemmed Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210
title_short Coupling of vesicle tethering and Rab binding is required for in vivo functionality of the golgin GMAP-210
title_sort coupling of vesicle tethering and rab binding is required for in vivo functionality of the golgin gmap-210
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310744/
https://www.ncbi.nlm.nih.gov/pubmed/25473115
http://dx.doi.org/10.1091/mbc.E14-10-1450
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