Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development

In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concen...

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Detalles Bibliográficos
Autores principales: Hu, Jie-Bi, Chen, Ting-Ru, Chen, Yu-Chie, Urban, Pawel L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311256/
https://www.ncbi.nlm.nih.gov/pubmed/25633338
http://dx.doi.org/10.1038/srep08135
Descripción
Sumario:In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2–48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.

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