Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development

In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concen...

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Autores principales: Hu, Jie-Bi, Chen, Ting-Ru, Chen, Yu-Chie, Urban, Pawel L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311256/
https://www.ncbi.nlm.nih.gov/pubmed/25633338
http://dx.doi.org/10.1038/srep08135
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author Hu, Jie-Bi
Chen, Ting-Ru
Chen, Yu-Chie
Urban, Pawel L.
author_facet Hu, Jie-Bi
Chen, Ting-Ru
Chen, Yu-Chie
Urban, Pawel L.
author_sort Hu, Jie-Bi
collection PubMed
description In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2–48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.
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spelling pubmed-43112562015-02-09 Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development Hu, Jie-Bi Chen, Ting-Ru Chen, Yu-Chie Urban, Pawel L. Sci Rep Article In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2–48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme. Nature Publishing Group 2015-01-30 /pmc/articles/PMC4311256/ /pubmed/25633338 http://dx.doi.org/10.1038/srep08135 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hu, Jie-Bi
Chen, Ting-Ru
Chen, Yu-Chie
Urban, Pawel L.
Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development
title Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development
title_full Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development
title_fullStr Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development
title_full_unstemmed Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development
title_short Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development
title_sort microcontroller-assisted compensation of adenosine triphosphate levels: instrument and method development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311256/
https://www.ncbi.nlm.nih.gov/pubmed/25633338
http://dx.doi.org/10.1038/srep08135
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