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Prevalence of Antibiotic Resistance Genes in Subjects with Successful and Failing Dental Implants. A Pilot Study

OBJECTIVES : To investigate the prevalence of the bacterial genes encoding resistance to beta-lactams, tetracyclines and metronidazole respectively, in subjects with successful and failing dental implants and to assess the presence of Staphylococcus aureus and the mecA gene encoding for Methicillin...

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Autores principales: Koukos, Georgios, Papadopoulos, Christos, Tsalikis, Lazaros, Sakellari, Dimitra, Arsenakis, Minas, Konstantinidis, Antonios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311380/
https://www.ncbi.nlm.nih.gov/pubmed/25646133
http://dx.doi.org/10.2174/1874210601408010257
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author Koukos, Georgios
Papadopoulos, Christos
Tsalikis, Lazaros
Sakellari, Dimitra
Arsenakis, Minas
Konstantinidis, Antonios
author_facet Koukos, Georgios
Papadopoulos, Christos
Tsalikis, Lazaros
Sakellari, Dimitra
Arsenakis, Minas
Konstantinidis, Antonios
author_sort Koukos, Georgios
collection PubMed
description OBJECTIVES : To investigate the prevalence of the bacterial genes encoding resistance to beta-lactams, tetracyclines and metronidazole respectively, in subjects with successful and failing dental implants and to assess the presence of Staphylococcus aureus and the mecA gene encoding for Methicillin Resistant Staphylococcus aureus (MRSA) in the same samples. MATERIALS AND METHODOLOGY: The subject sample included 20 participants with clinically healthy osseointegrated implants and 20 participants with implants exhibiting peri-implantitis. Clinical parameters were assessed with an automated probe, samples were collected from the peri-implant sulcus or pocket and analyzed with Polymerase Chain Reaction for bla(TEM), tetM, tetQ and nim genes, S. aureus and MRSA using primers and conditions previously described in the literature. RESULTS: Findings have shown high frequencies of detection for both groups for the tetracycline resistance genes tetM (>30%), tetQ (>65%) with no statistical differences between them (z-test with Bonferroni corrections, p<0.05). The bla(TEM )gene, which encodes resistance to beta-lactams, was detected in <15% of the samples. The nim gene, which encodes resistance to metronidazole, S.aureus and the mecA gene encoding for MRSA were not detected in any of the analyzed samples. CONCLUSIONS: Healthy peri-implant sulci and peri-implantitis cases often harbor bacterial genes encoding for resistance to the tetracyclines and less often for beta-lactams. Thus, the antimicrobial activity of the tetracyclines and to a lower extent to beta-lactams, might be compromised for treatment of peri-implantitis. Since no metronidazole resistance genes were detected in the present study, its clinical use is supported by the current findings. S.aureus may not participate in peri-implant pathology.
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spelling pubmed-43113802015-02-02 Prevalence of Antibiotic Resistance Genes in Subjects with Successful and Failing Dental Implants. A Pilot Study Koukos, Georgios Papadopoulos, Christos Tsalikis, Lazaros Sakellari, Dimitra Arsenakis, Minas Konstantinidis, Antonios Open Dent J Article OBJECTIVES : To investigate the prevalence of the bacterial genes encoding resistance to beta-lactams, tetracyclines and metronidazole respectively, in subjects with successful and failing dental implants and to assess the presence of Staphylococcus aureus and the mecA gene encoding for Methicillin Resistant Staphylococcus aureus (MRSA) in the same samples. MATERIALS AND METHODOLOGY: The subject sample included 20 participants with clinically healthy osseointegrated implants and 20 participants with implants exhibiting peri-implantitis. Clinical parameters were assessed with an automated probe, samples were collected from the peri-implant sulcus or pocket and analyzed with Polymerase Chain Reaction for bla(TEM), tetM, tetQ and nim genes, S. aureus and MRSA using primers and conditions previously described in the literature. RESULTS: Findings have shown high frequencies of detection for both groups for the tetracycline resistance genes tetM (>30%), tetQ (>65%) with no statistical differences between them (z-test with Bonferroni corrections, p<0.05). The bla(TEM )gene, which encodes resistance to beta-lactams, was detected in <15% of the samples. The nim gene, which encodes resistance to metronidazole, S.aureus and the mecA gene encoding for MRSA were not detected in any of the analyzed samples. CONCLUSIONS: Healthy peri-implant sulci and peri-implantitis cases often harbor bacterial genes encoding for resistance to the tetracyclines and less often for beta-lactams. Thus, the antimicrobial activity of the tetracyclines and to a lower extent to beta-lactams, might be compromised for treatment of peri-implantitis. Since no metronidazole resistance genes were detected in the present study, its clinical use is supported by the current findings. S.aureus may not participate in peri-implant pathology. Bentham Open 2015-01-6 /pmc/articles/PMC4311380/ /pubmed/25646133 http://dx.doi.org/10.2174/1874210601408010257 Text en © Koukos et al.; Licensee Bentham Open. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Koukos, Georgios
Papadopoulos, Christos
Tsalikis, Lazaros
Sakellari, Dimitra
Arsenakis, Minas
Konstantinidis, Antonios
Prevalence of Antibiotic Resistance Genes in Subjects with Successful and Failing Dental Implants. A Pilot Study
title Prevalence of Antibiotic Resistance Genes in Subjects with Successful and Failing Dental Implants. A Pilot Study
title_full Prevalence of Antibiotic Resistance Genes in Subjects with Successful and Failing Dental Implants. A Pilot Study
title_fullStr Prevalence of Antibiotic Resistance Genes in Subjects with Successful and Failing Dental Implants. A Pilot Study
title_full_unstemmed Prevalence of Antibiotic Resistance Genes in Subjects with Successful and Failing Dental Implants. A Pilot Study
title_short Prevalence of Antibiotic Resistance Genes in Subjects with Successful and Failing Dental Implants. A Pilot Study
title_sort prevalence of antibiotic resistance genes in subjects with successful and failing dental implants. a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311380/
https://www.ncbi.nlm.nih.gov/pubmed/25646133
http://dx.doi.org/10.2174/1874210601408010257
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