Tracking down the sources of experimental contamination in microbiome studies

A recent report warns that DNA extraction kits and other laboratory reagents are considerable sources of contamination in microbiome experiments. The issue of contamination is particularly problematic for samples of low biomass. See related research, http://www.biomedcentral.com/1741-7007/12/87

Detalles Bibliográficos
Autores principales: Weiss, Sophie, Amir, Amnon, Hyde, Embriette R, Metcalf, Jessica L, Song, Se Jin, Knight, Rob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research Highlight
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311479/
https://www.ncbi.nlm.nih.gov/pubmed/25608874
http://dx.doi.org/10.1186/s13059-014-0564-2
_version_ 1782355004648062976
author Weiss, Sophie
Amir, Amnon
Hyde, Embriette R
Metcalf, Jessica L
Song, Se Jin
Knight, Rob
author_facet Weiss, Sophie
Amir, Amnon
Hyde, Embriette R
Metcalf, Jessica L
Song, Se Jin
Knight, Rob
author_sort Weiss, Sophie
collection PubMed
description A recent report warns that DNA extraction kits and other laboratory reagents are considerable sources of contamination in microbiome experiments. The issue of contamination is particularly problematic for samples of low biomass. See related research, http://www.biomedcentral.com/1741-7007/12/87
format Online
Article
Text
id pubmed-4311479
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-43114792015-01-31 Tracking down the sources of experimental contamination in microbiome studies Weiss, Sophie Amir, Amnon Hyde, Embriette R Metcalf, Jessica L Song, Se Jin Knight, Rob Genome Biol Research Highlight A recent report warns that DNA extraction kits and other laboratory reagents are considerable sources of contamination in microbiome experiments. The issue of contamination is particularly problematic for samples of low biomass. See related research, http://www.biomedcentral.com/1741-7007/12/87 BioMed Central 2014-12-17 2014 /pmc/articles/PMC4311479/ /pubmed/25608874 http://dx.doi.org/10.1186/s13059-014-0564-2 Text en © Weiss et al.; licensee BioMed Central Ltd. 2014 The licensee has exclusive rights to distribute this article, in any medium, for 12 months following its publication. After this time, the article is available under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Highlight
Weiss, Sophie
Amir, Amnon
Hyde, Embriette R
Metcalf, Jessica L
Song, Se Jin
Knight, Rob
Tracking down the sources of experimental contamination in microbiome studies
title Tracking down the sources of experimental contamination in microbiome studies
title_full Tracking down the sources of experimental contamination in microbiome studies
title_fullStr Tracking down the sources of experimental contamination in microbiome studies
title_full_unstemmed Tracking down the sources of experimental contamination in microbiome studies
title_short Tracking down the sources of experimental contamination in microbiome studies
title_sort tracking down the sources of experimental contamination in microbiome studies
topic Research Highlight
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311479/
https://www.ncbi.nlm.nih.gov/pubmed/25608874
http://dx.doi.org/10.1186/s13059-014-0564-2
work_keys_str_mv AT weisssophie trackingdownthesourcesofexperimentalcontaminationinmicrobiomestudies
AT amiramnon trackingdownthesourcesofexperimentalcontaminationinmicrobiomestudies
AT hydeembrietter trackingdownthesourcesofexperimentalcontaminationinmicrobiomestudies
AT metcalfjessical trackingdownthesourcesofexperimentalcontaminationinmicrobiomestudies
AT songsejin trackingdownthesourcesofexperimentalcontaminationinmicrobiomestudies
AT knightrob trackingdownthesourcesofexperimentalcontaminationinmicrobiomestudies

Ejemplares similares