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Characterization of miRNAs associated with Botrytis cinerea infection of tomato leaves
BACKGROUND: Botrytis cinerea Pers. Fr. is an important pathogen causing stem rot in tomatoes grown indoors for extended periods. MicroRNAs (miRNAs) have been reported as gene expression regulators related to several stress responses and B. cinerea infection in tomato. However, the function of miRNAs...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311480/ https://www.ncbi.nlm.nih.gov/pubmed/25592487 http://dx.doi.org/10.1186/s12870-014-0410-4 |
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author | Jin, Weibo Wu, Fangli |
author_facet | Jin, Weibo Wu, Fangli |
author_sort | Jin, Weibo |
collection | PubMed |
description | BACKGROUND: Botrytis cinerea Pers. Fr. is an important pathogen causing stem rot in tomatoes grown indoors for extended periods. MicroRNAs (miRNAs) have been reported as gene expression regulators related to several stress responses and B. cinerea infection in tomato. However, the function of miRNAs in the resistance to B. cinerea remains unclear. RESULTS: The miRNA expression patterns in tomato in response to B. cinerea stress were investigated by high-throughput sequencing. In total, 143 known miRNAs and seven novel miRNAs were identified and their corresponding expression was detected in mock- and B. cinerea-inoculated leaves. Among those, one novel and 57 known miRNAs were differentially expressed in B. cinerea-infected leaves, and 8 of these were further confirmed by quantitative reverse-transcription PCR (qRT-PCR). Moreover, five of these eight differentially expressed miRNAs could hit 10 coding sequences (CDSs) via CleaveLand pipeline and psRNAtarget program. In addition, qRT-PCR revealed that four targets were negatively correlated with their corresponding miRNAs (miR319, miR394, and miRn1). CONCLUSION: Results of sRNA high-throughput sequencing revealed that the upregulation of miRNAs may be implicated in the mechanism by which tomato respond to B. cinerea stress. Analysis of the expression profiles of B. cinerea-responsive miRNAs and their targets strongly suggested that miR319, miR394, and miRn1 may be involved in the tomato leaves’ response to B. cinerea infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0410-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4311480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43114802015-01-31 Characterization of miRNAs associated with Botrytis cinerea infection of tomato leaves Jin, Weibo Wu, Fangli BMC Plant Biol Research Article BACKGROUND: Botrytis cinerea Pers. Fr. is an important pathogen causing stem rot in tomatoes grown indoors for extended periods. MicroRNAs (miRNAs) have been reported as gene expression regulators related to several stress responses and B. cinerea infection in tomato. However, the function of miRNAs in the resistance to B. cinerea remains unclear. RESULTS: The miRNA expression patterns in tomato in response to B. cinerea stress were investigated by high-throughput sequencing. In total, 143 known miRNAs and seven novel miRNAs were identified and their corresponding expression was detected in mock- and B. cinerea-inoculated leaves. Among those, one novel and 57 known miRNAs were differentially expressed in B. cinerea-infected leaves, and 8 of these were further confirmed by quantitative reverse-transcription PCR (qRT-PCR). Moreover, five of these eight differentially expressed miRNAs could hit 10 coding sequences (CDSs) via CleaveLand pipeline and psRNAtarget program. In addition, qRT-PCR revealed that four targets were negatively correlated with their corresponding miRNAs (miR319, miR394, and miRn1). CONCLUSION: Results of sRNA high-throughput sequencing revealed that the upregulation of miRNAs may be implicated in the mechanism by which tomato respond to B. cinerea stress. Analysis of the expression profiles of B. cinerea-responsive miRNAs and their targets strongly suggested that miR319, miR394, and miRn1 may be involved in the tomato leaves’ response to B. cinerea infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0410-4) contains supplementary material, which is available to authorized users. BioMed Central 2015-01-16 /pmc/articles/PMC4311480/ /pubmed/25592487 http://dx.doi.org/10.1186/s12870-014-0410-4 Text en © Jin and Wu; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Jin, Weibo Wu, Fangli Characterization of miRNAs associated with Botrytis cinerea infection of tomato leaves |
title | Characterization of miRNAs associated with Botrytis cinerea infection of tomato leaves |
title_full | Characterization of miRNAs associated with Botrytis cinerea infection of tomato leaves |
title_fullStr | Characterization of miRNAs associated with Botrytis cinerea infection of tomato leaves |
title_full_unstemmed | Characterization of miRNAs associated with Botrytis cinerea infection of tomato leaves |
title_short | Characterization of miRNAs associated with Botrytis cinerea infection of tomato leaves |
title_sort | characterization of mirnas associated with botrytis cinerea infection of tomato leaves |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311480/ https://www.ncbi.nlm.nih.gov/pubmed/25592487 http://dx.doi.org/10.1186/s12870-014-0410-4 |
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