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Effect of curcumin on rat sperm morphology after the freeze-thawing process

Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viabili...

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Detalles Bibliográficos
Autores principales: Soleimanzadeh, Ali, Saberivand, Adel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312379/
https://www.ncbi.nlm.nih.gov/pubmed/25653795
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author Soleimanzadeh, Ali
Saberivand, Adel
author_facet Soleimanzadeh, Ali
Saberivand, Adel
author_sort Soleimanzadeh, Ali
collection PubMed
description Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-thawing process. Sperm collected from 10 adult rats were divided into two groups (n=10 for each group): control and a test group supplemented with 2.5 mM curcumin. After freezing-thawing, the number of spermatozoa, motility, viability, total antioxidant capacity (TAC), and DNA integrity of the sperm were analyzed. Motility, viability and DNA integrity of sperm were significantly preserved in treatment groups compared to the control (p < 0.05) after freezing-thawing. Following cryopreservation, TAC was significantly preserved in thawing semen supplemented with curcumin compared to the control group (p<0.05). Based on our results, it is concluded that curcumin addition during freezing resulted in positive effects on sperm parameters after thawing in adult rats.
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spelling pubmed-43123792015-02-04 Effect of curcumin on rat sperm morphology after the freeze-thawing process Soleimanzadeh, Ali Saberivand, Adel Vet Res Forum Original Article Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-thawing process. Sperm collected from 10 adult rats were divided into two groups (n=10 for each group): control and a test group supplemented with 2.5 mM curcumin. After freezing-thawing, the number of spermatozoa, motility, viability, total antioxidant capacity (TAC), and DNA integrity of the sperm were analyzed. Motility, viability and DNA integrity of sperm were significantly preserved in treatment groups compared to the control (p < 0.05) after freezing-thawing. Following cryopreservation, TAC was significantly preserved in thawing semen supplemented with curcumin compared to the control group (p<0.05). Based on our results, it is concluded that curcumin addition during freezing resulted in positive effects on sperm parameters after thawing in adult rats. Urmia University Press 2013 /pmc/articles/PMC4312379/ /pubmed/25653795 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Soleimanzadeh, Ali
Saberivand, Adel
Effect of curcumin on rat sperm morphology after the freeze-thawing process
title Effect of curcumin on rat sperm morphology after the freeze-thawing process
title_full Effect of curcumin on rat sperm morphology after the freeze-thawing process
title_fullStr Effect of curcumin on rat sperm morphology after the freeze-thawing process
title_full_unstemmed Effect of curcumin on rat sperm morphology after the freeze-thawing process
title_short Effect of curcumin on rat sperm morphology after the freeze-thawing process
title_sort effect of curcumin on rat sperm morphology after the freeze-thawing process
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312379/
https://www.ncbi.nlm.nih.gov/pubmed/25653795
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