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The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide
BACKGROUND: Antimicrobial resistance continues to be a global issue in healthcare organisations. Honey has long been shown to possess wound healing and antimicrobial properties that are dependent on a number of physical and chemical properties of the honey. We tested the antimicrobial activity of a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312449/ https://www.ncbi.nlm.nih.gov/pubmed/25627827 http://dx.doi.org/10.1186/s13104-014-0960-4 |
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author | Cooke, Jonathan Dryden, Matthew Patton, Thomas Brennan, James Barrett, John |
author_facet | Cooke, Jonathan Dryden, Matthew Patton, Thomas Brennan, James Barrett, John |
author_sort | Cooke, Jonathan |
collection | PubMed |
description | BACKGROUND: Antimicrobial resistance continues to be a global issue in healthcare organisations. Honey has long been shown to possess wound healing and antimicrobial properties that are dependent on a number of physical and chemical properties of the honey. We tested the antimicrobial activity of a medicinal honey, Surgihoney® (SH) and two prototype modified honeys made by Apis mellifera (honeybee) against Staphylococcus aureus (NCIMB 9518). We also examined the modified honey prototypes for the ability to generate reactive oxygen species (ROS) by changing the level of production of hydrogen peroxide from the samples. METHODS: Surgihoney® (SH) was compared with two modified honeys, Prototype 1 (PT1) and Prototype 2 (PT2) using a bioassay method against a standard strain of Staphylococcus aureus. Further work studied the rate of generation of ROS hydrogen peroxide from these preparations. RESULTS: Surgihoney® antimicrobial activity was shown to be largely due to ROS hydrogen peroxide production. By modification of Surgihoney®, two more potent honey prototypes were shown to generate between a two- and three-fold greater antibacterial activity and up to ten times greater ROS peroxide activity. CONCLUSIONS: Surgihoney® is a clinically available wound antiseptic dressing that shows good antimicrobial activity. Two further honey prototypes have been shown to have antimicrobial activity that is possible to be enhanced due to demonstrated increases in ROS peroxide activity. |
format | Online Article Text |
id | pubmed-4312449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43124492015-02-03 The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide Cooke, Jonathan Dryden, Matthew Patton, Thomas Brennan, James Barrett, John BMC Res Notes Research Article BACKGROUND: Antimicrobial resistance continues to be a global issue in healthcare organisations. Honey has long been shown to possess wound healing and antimicrobial properties that are dependent on a number of physical and chemical properties of the honey. We tested the antimicrobial activity of a medicinal honey, Surgihoney® (SH) and two prototype modified honeys made by Apis mellifera (honeybee) against Staphylococcus aureus (NCIMB 9518). We also examined the modified honey prototypes for the ability to generate reactive oxygen species (ROS) by changing the level of production of hydrogen peroxide from the samples. METHODS: Surgihoney® (SH) was compared with two modified honeys, Prototype 1 (PT1) and Prototype 2 (PT2) using a bioassay method against a standard strain of Staphylococcus aureus. Further work studied the rate of generation of ROS hydrogen peroxide from these preparations. RESULTS: Surgihoney® antimicrobial activity was shown to be largely due to ROS hydrogen peroxide production. By modification of Surgihoney®, two more potent honey prototypes were shown to generate between a two- and three-fold greater antibacterial activity and up to ten times greater ROS peroxide activity. CONCLUSIONS: Surgihoney® is a clinically available wound antiseptic dressing that shows good antimicrobial activity. Two further honey prototypes have been shown to have antimicrobial activity that is possible to be enhanced due to demonstrated increases in ROS peroxide activity. BioMed Central 2015-01-28 /pmc/articles/PMC4312449/ /pubmed/25627827 http://dx.doi.org/10.1186/s13104-014-0960-4 Text en © Cooke et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Cooke, Jonathan Dryden, Matthew Patton, Thomas Brennan, James Barrett, John The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide |
title | The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide |
title_full | The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide |
title_fullStr | The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide |
title_full_unstemmed | The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide |
title_short | The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide |
title_sort | antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ros) hydrogen peroxide |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312449/ https://www.ncbi.nlm.nih.gov/pubmed/25627827 http://dx.doi.org/10.1186/s13104-014-0960-4 |
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