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Isoform Composition and Gene Expression of Thick and Thin Filament Proteins in Striated Muscles of Mice after 30-Day Space Flight

Changes in isoform composition, gene expression of titin and nebulin, and isoform composition of myosin heavy chains as well as changes in titin phosphorylation level in skeletal (m. gastrocnemius, m. tibialis anterior, and m. psoas) and cardiac muscles of mice were studied after a 30-day-long space...

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Autores principales: Ulanova, Anna, Gritsyna, Yulia, Vikhlyantsev, Ivan, Salmov, Nikolay, Bobylev, Alexander, Abdusalamova, Zarema, Rogachevsky, Vadim, Shenkman, Boris, Podlubnaya, Zoya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312622/
https://www.ncbi.nlm.nih.gov/pubmed/25664316
http://dx.doi.org/10.1155/2015/104735
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author Ulanova, Anna
Gritsyna, Yulia
Vikhlyantsev, Ivan
Salmov, Nikolay
Bobylev, Alexander
Abdusalamova, Zarema
Rogachevsky, Vadim
Shenkman, Boris
Podlubnaya, Zoya
author_facet Ulanova, Anna
Gritsyna, Yulia
Vikhlyantsev, Ivan
Salmov, Nikolay
Bobylev, Alexander
Abdusalamova, Zarema
Rogachevsky, Vadim
Shenkman, Boris
Podlubnaya, Zoya
author_sort Ulanova, Anna
collection PubMed
description Changes in isoform composition, gene expression of titin and nebulin, and isoform composition of myosin heavy chains as well as changes in titin phosphorylation level in skeletal (m. gastrocnemius, m. tibialis anterior, and m. psoas) and cardiac muscles of mice were studied after a 30-day-long space flight onboard the Russian spacecraft “BION-M” number 1. A muscle fibre-type shift from slow-to-fast and a decrease in the content of titin and nebulin in the skeletal muscles of animals from “Flight” group was found. Using Pro-Q Diamond staining, an ~3-fold increase in the phosphorylation level of titin in m. gastrocnemius of mice from the “Flight” group was detected. The content of titin and its phosphorylation level in the cardiac muscle of mice from “Flight” and “Control” groups did not differ; nevertheless an increase (2.2 times) in titin gene expression in the myocardium of flight animals was found. The observed changes are discussed in the context of their role in the contractile activity of striated muscles of mice under conditions of weightlessness.
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spelling pubmed-43126222015-02-08 Isoform Composition and Gene Expression of Thick and Thin Filament Proteins in Striated Muscles of Mice after 30-Day Space Flight Ulanova, Anna Gritsyna, Yulia Vikhlyantsev, Ivan Salmov, Nikolay Bobylev, Alexander Abdusalamova, Zarema Rogachevsky, Vadim Shenkman, Boris Podlubnaya, Zoya Biomed Res Int Research Article Changes in isoform composition, gene expression of titin and nebulin, and isoform composition of myosin heavy chains as well as changes in titin phosphorylation level in skeletal (m. gastrocnemius, m. tibialis anterior, and m. psoas) and cardiac muscles of mice were studied after a 30-day-long space flight onboard the Russian spacecraft “BION-M” number 1. A muscle fibre-type shift from slow-to-fast and a decrease in the content of titin and nebulin in the skeletal muscles of animals from “Flight” group was found. Using Pro-Q Diamond staining, an ~3-fold increase in the phosphorylation level of titin in m. gastrocnemius of mice from the “Flight” group was detected. The content of titin and its phosphorylation level in the cardiac muscle of mice from “Flight” and “Control” groups did not differ; nevertheless an increase (2.2 times) in titin gene expression in the myocardium of flight animals was found. The observed changes are discussed in the context of their role in the contractile activity of striated muscles of mice under conditions of weightlessness. Hindawi Publishing Corporation 2015 2015-01-18 /pmc/articles/PMC4312622/ /pubmed/25664316 http://dx.doi.org/10.1155/2015/104735 Text en Copyright © 2015 Anna Ulanova et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ulanova, Anna
Gritsyna, Yulia
Vikhlyantsev, Ivan
Salmov, Nikolay
Bobylev, Alexander
Abdusalamova, Zarema
Rogachevsky, Vadim
Shenkman, Boris
Podlubnaya, Zoya
Isoform Composition and Gene Expression of Thick and Thin Filament Proteins in Striated Muscles of Mice after 30-Day Space Flight
title Isoform Composition and Gene Expression of Thick and Thin Filament Proteins in Striated Muscles of Mice after 30-Day Space Flight
title_full Isoform Composition and Gene Expression of Thick and Thin Filament Proteins in Striated Muscles of Mice after 30-Day Space Flight
title_fullStr Isoform Composition and Gene Expression of Thick and Thin Filament Proteins in Striated Muscles of Mice after 30-Day Space Flight
title_full_unstemmed Isoform Composition and Gene Expression of Thick and Thin Filament Proteins in Striated Muscles of Mice after 30-Day Space Flight
title_short Isoform Composition and Gene Expression of Thick and Thin Filament Proteins in Striated Muscles of Mice after 30-Day Space Flight
title_sort isoform composition and gene expression of thick and thin filament proteins in striated muscles of mice after 30-day space flight
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312622/
https://www.ncbi.nlm.nih.gov/pubmed/25664316
http://dx.doi.org/10.1155/2015/104735
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