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Immunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins
Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chr...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Urmia University Press
2012
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313042/ https://www.ncbi.nlm.nih.gov/pubmed/25653765 |