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Improvement of DNA vaccination by adjuvants and sophisticated delivery devices: vaccine-platforms for the battle against infectious diseases

Advantages of DNA vaccination against infectious diseases over more classical immunization methods include the possibilities for rapid manufacture, fast adaptation to newly emerging pathogens and high stability at ambient temperatures. In addition, upon DNA immunization the antigen is produced by th...

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Detalles Bibliográficos
Autores principales: Grunwald, Thomas, Ulbert, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Vaccine Society 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313101/
https://www.ncbi.nlm.nih.gov/pubmed/25648133
http://dx.doi.org/10.7774/cevr.2015.4.1.1
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author Grunwald, Thomas
Ulbert, Sebastian
author_facet Grunwald, Thomas
Ulbert, Sebastian
author_sort Grunwald, Thomas
collection PubMed
description Advantages of DNA vaccination against infectious diseases over more classical immunization methods include the possibilities for rapid manufacture, fast adaptation to newly emerging pathogens and high stability at ambient temperatures. In addition, upon DNA immunization the antigen is produced by the cells of the vaccinated individual, which leads to activation of both cellular and humoral immune responses due to antigen presentation via MHC I and MHC II molecules. However, so far DNA vaccines have shown most efficient immunogenicity mainly in small rodent models, whereas in larger animals including humans there is still the need to improve effectiveness. This is mostly due to inefficient delivery of the DNA plasmid into cells and nuclei. Here, we discuss technologies used to overcome this problem, including physical means such as in vivo electroporation and co-administration of adjuvants. Several of these methods have already entered clinical testing in humans.
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spelling pubmed-43131012015-02-03 Improvement of DNA vaccination by adjuvants and sophisticated delivery devices: vaccine-platforms for the battle against infectious diseases Grunwald, Thomas Ulbert, Sebastian Clin Exp Vaccine Res Special Article Advantages of DNA vaccination against infectious diseases over more classical immunization methods include the possibilities for rapid manufacture, fast adaptation to newly emerging pathogens and high stability at ambient temperatures. In addition, upon DNA immunization the antigen is produced by the cells of the vaccinated individual, which leads to activation of both cellular and humoral immune responses due to antigen presentation via MHC I and MHC II molecules. However, so far DNA vaccines have shown most efficient immunogenicity mainly in small rodent models, whereas in larger animals including humans there is still the need to improve effectiveness. This is mostly due to inefficient delivery of the DNA plasmid into cells and nuclei. Here, we discuss technologies used to overcome this problem, including physical means such as in vivo electroporation and co-administration of adjuvants. Several of these methods have already entered clinical testing in humans. The Korean Vaccine Society 2015-01 2015-01-30 /pmc/articles/PMC4313101/ /pubmed/25648133 http://dx.doi.org/10.7774/cevr.2015.4.1.1 Text en © Korean Vaccine Society. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Special Article
Grunwald, Thomas
Ulbert, Sebastian
Improvement of DNA vaccination by adjuvants and sophisticated delivery devices: vaccine-platforms for the battle against infectious diseases
title Improvement of DNA vaccination by adjuvants and sophisticated delivery devices: vaccine-platforms for the battle against infectious diseases
title_full Improvement of DNA vaccination by adjuvants and sophisticated delivery devices: vaccine-platforms for the battle against infectious diseases
title_fullStr Improvement of DNA vaccination by adjuvants and sophisticated delivery devices: vaccine-platforms for the battle against infectious diseases
title_full_unstemmed Improvement of DNA vaccination by adjuvants and sophisticated delivery devices: vaccine-platforms for the battle against infectious diseases
title_short Improvement of DNA vaccination by adjuvants and sophisticated delivery devices: vaccine-platforms for the battle against infectious diseases
title_sort improvement of dna vaccination by adjuvants and sophisticated delivery devices: vaccine-platforms for the battle against infectious diseases
topic Special Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313101/
https://www.ncbi.nlm.nih.gov/pubmed/25648133
http://dx.doi.org/10.7774/cevr.2015.4.1.1
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