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Comparative evaluation of booster efficacies of BCG, Ag85B, and Ag85B peptides based vaccines to boost BCG induced immunity in BALB/c mice: a pilot study

PURPOSE: In the present study booster efficacies of Ag85 B, Bacillus Calmette-Guerin (BCG), and Ag85B peptides were evaluated using prime boost regimes in BALB/c mice. MATERIALS AND METHODS: Mice were primed with BCG vaccine and subsequently boosted with Ag85B, BCG and cocktail of Ag85B peptides. RE...

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Autores principales: Husain, Aliabbas A., Warke, Shubhangi R., Kalorey, Dewanand R., Daginawala, Hatim F., Taori, Girdhar M., Kashyap, Rajpal S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Vaccine Society 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313113/
https://www.ncbi.nlm.nih.gov/pubmed/25649326
http://dx.doi.org/10.7774/cevr.2015.4.1.83
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author Husain, Aliabbas A.
Warke, Shubhangi R.
Kalorey, Dewanand R.
Daginawala, Hatim F.
Taori, Girdhar M.
Kashyap, Rajpal S.
author_facet Husain, Aliabbas A.
Warke, Shubhangi R.
Kalorey, Dewanand R.
Daginawala, Hatim F.
Taori, Girdhar M.
Kashyap, Rajpal S.
author_sort Husain, Aliabbas A.
collection PubMed
description PURPOSE: In the present study booster efficacies of Ag85 B, Bacillus Calmette-Guerin (BCG), and Ag85B peptides were evaluated using prime boost regimes in BALB/c mice. MATERIALS AND METHODS: Mice were primed with BCG vaccine and subsequently boosted with Ag85B, BCG and cocktail of Ag85B peptides. RESULTS: Based on analysis of immune response it was observed mice boosted with Ag85B peptides showed significant (p < 0.001) cytokines levels (interferon γ, interleukin 12) and BCG specific antibodies (anti-BCG and anti-purified protein derivative titre) compared to booster dose of BCG, Ag85B and BCG alone. CONCLUSION: Our pilot results suggest that prime boost regimes with Ag85B peptides can boost waning BCG induced immunity and may improve immunogenicity of BCG vaccine. However, lot of work is further needed using experimental model of tuberculosis infection to justify the result.
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spelling pubmed-43131132015-02-03 Comparative evaluation of booster efficacies of BCG, Ag85B, and Ag85B peptides based vaccines to boost BCG induced immunity in BALB/c mice: a pilot study Husain, Aliabbas A. Warke, Shubhangi R. Kalorey, Dewanand R. Daginawala, Hatim F. Taori, Girdhar M. Kashyap, Rajpal S. Clin Exp Vaccine Res Original Article PURPOSE: In the present study booster efficacies of Ag85 B, Bacillus Calmette-Guerin (BCG), and Ag85B peptides were evaluated using prime boost regimes in BALB/c mice. MATERIALS AND METHODS: Mice were primed with BCG vaccine and subsequently boosted with Ag85B, BCG and cocktail of Ag85B peptides. RESULTS: Based on analysis of immune response it was observed mice boosted with Ag85B peptides showed significant (p < 0.001) cytokines levels (interferon γ, interleukin 12) and BCG specific antibodies (anti-BCG and anti-purified protein derivative titre) compared to booster dose of BCG, Ag85B and BCG alone. CONCLUSION: Our pilot results suggest that prime boost regimes with Ag85B peptides can boost waning BCG induced immunity and may improve immunogenicity of BCG vaccine. However, lot of work is further needed using experimental model of tuberculosis infection to justify the result. The Korean Vaccine Society 2015-01 2015-01-30 /pmc/articles/PMC4313113/ /pubmed/25649326 http://dx.doi.org/10.7774/cevr.2015.4.1.83 Text en © Korean Vaccine Society. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Husain, Aliabbas A.
Warke, Shubhangi R.
Kalorey, Dewanand R.
Daginawala, Hatim F.
Taori, Girdhar M.
Kashyap, Rajpal S.
Comparative evaluation of booster efficacies of BCG, Ag85B, and Ag85B peptides based vaccines to boost BCG induced immunity in BALB/c mice: a pilot study
title Comparative evaluation of booster efficacies of BCG, Ag85B, and Ag85B peptides based vaccines to boost BCG induced immunity in BALB/c mice: a pilot study
title_full Comparative evaluation of booster efficacies of BCG, Ag85B, and Ag85B peptides based vaccines to boost BCG induced immunity in BALB/c mice: a pilot study
title_fullStr Comparative evaluation of booster efficacies of BCG, Ag85B, and Ag85B peptides based vaccines to boost BCG induced immunity in BALB/c mice: a pilot study
title_full_unstemmed Comparative evaluation of booster efficacies of BCG, Ag85B, and Ag85B peptides based vaccines to boost BCG induced immunity in BALB/c mice: a pilot study
title_short Comparative evaluation of booster efficacies of BCG, Ag85B, and Ag85B peptides based vaccines to boost BCG induced immunity in BALB/c mice: a pilot study
title_sort comparative evaluation of booster efficacies of bcg, ag85b, and ag85b peptides based vaccines to boost bcg induced immunity in balb/c mice: a pilot study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313113/
https://www.ncbi.nlm.nih.gov/pubmed/25649326
http://dx.doi.org/10.7774/cevr.2015.4.1.83
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